Evaluation of Antibacterial Activity of Tetmosol® against some Clinical Isolates

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Publié-e : août 23, 2020
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Evaluation of antibacterial, Activity, Tetmosol®,, Disinfectant, Clinical isolates

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Clement O. Anie
Johnson D. Jemikalajah
Merit O. Meshack
Oghenejobo Michael

Résumé

The study was carried out to evaluate the antibacterial activities of Tetmosol® using some clinical bacterial isolates. The broth dilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC). The result indicated that the sample of tetmosol was effective as a disinfectant with broad spectrum of activity. The MIC of Tetmosol using broth technique was 1.2125 %v/v, 0.60625 %v/v, O.60625 %v/v, 0.60625 %v/v for Staphylococcus aureus, Streptococcus pyogene, Escherichia coli, Pseudomonas aeruginosa respectively. The MBC using agar technique was 6% and that of broth technique 9%. Susceptibility testing of the microorganism to Tetmosol, showed the highest zones of inhibition were 11 mm,14 mm, 12 mm, 10 mm for Staphylococcus aureus, Streptococcus pyogene, Escherichia coli, Pseudomonas aeruginosa respectively. The phenol coefficient of Tetmosol was found to be 9.1 which indicated that Tetmosol was more potent than phenol. The use of Tetmosol® in hospital, water bath and clinics in Nigeria is recommended.

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Anie, C. O. ., Jemikalajah, J. D. ., Meshack, M. O. ., & Michael, O. (2020). Evaluation of Antibacterial Activity of Tetmosol® against some Clinical Isolates. Nigerian Journal of Pharmaceutical and Applied Science Research, 9(1), 63–68. Consulté à l’adresse https://nijophasr.net/index.php/nijophasr/article/view/335
Numéro
Vol. 9 No. 1 (2020)
Rubrique
Articles
Licence Creative Commons

Cette œuvre est sous licence Creative Commons Attribution - Pas d'Utilisation Commerciale - Pas de Modification 4.0 International.

Bibliographies de l'auteur-e

Clement O. Anie

Department Of Pharmaceutical Microbiology, Faculty Of Pharmacy, Delta State University, Abraka, Delta State, Nigeria

Johnson D. Jemikalajah

Department of Microbiology, Faculty of Science Delta State University Abraka, Delta State, Nigeria.

Merit O. Meshack

Department Of Pharmaceutical Microbiology, Faculty Of Pharmacy, Delta State University, Abraka, Delta State, Nigeria

Oghenejobo Michael

Department Of Pharmaceutical Microbiology, Faculty Of Pharmacy, Delta State University, Abraka, Delta State, Nigeria

Références

Walsh SE, Maillard j, Russel AD, Catrenich CE, Chabanneau DL,Bratolo RG(2003).Development of bacterial resistance to several biocides and effects on antibiotic susceptibility J Hosp Infect. 55(2):98-107.

Block SS (1991).Disinfection, sterilization and preservation (4th Edn.)Lea and Febiger.

Block SS (2001). Disinfection, sterilization and preservation"(5th Edn.) Lippincott Williams and Wilkins Philadelphia and London.

Akimitsu N,Hamamoto H, Inoue RI, shoji M,Akamine A,Takemorikl, Hamasaki N, sekimizu K (1999). Increase in Resistance of methicillin-resistant Staphylococcus aureus to B-lactamams caused by mutation conferring resistance to benzaalkonium chloride, a disinfectant widely used in hospitals " Antimicrobial Agents chemotherapy 43:3042-3043

Rutala WA (1997).Disinfection, sterilization and waste disposal prevention and control of Nosocomial infections. wenzel R.P.,ed. Baltimore: Williams and wilkins. 539-93.

Joswick HL, Corner TR, Silveenale J N, Gerhardt P (1971) - Antimicrobial actions of hexachlorophene: release of cytoplasmic materials. Journal of Bacteriology. 108: 492-500

Russell AD (2004). Factors influencing the efficacy of germicides. In: Rutala WA, ed. Disinfection, sterilization and antisepsis: Principles, practices, challenges, and new research. Washington DC: Association for Professionals in Infection Control and Epidemiology.162-70.

Gillis RJ, Schmidt WC(1983). Scanning electron microscopy of spores on inoculated product surfaces.MD 1983:46-9.MD:46-9

Russell AD(1998). Bacterial resistance to disinfectants: Present knowledge and future problems. J. Hosp. Infect. 43:S57-S68

Russell AD, Russell NJ (1995). Biocides: activity, action and resistance. In: Hunter PA, Darby GK, Russell NJ, eds. Fifty years of antimicrobials: past perspectives and future trends. England: Cambridge University Press, 327-65.

Rutala WA, Cole EC (1987). Ineffectiveness of hospital disinfectants against bacteria: a collaborative study. Infect. Control 8:501-6.

Russell AD, McDonnell G. (2000). Concentration: a major factor in studying biocidal action. J. Hosp. Infect. 44:1-3.

Rutala W.A (1999) Selection and use of disinfectants in healthcare. In: Mayhall CG, ed. Infect. Control and Hosp. Epidemiol. Philadelphia: Lippincott Williams & Wilkins,1161-87.

Lewis D.L, Arens M(1995). Resistance of microorganisms to disinfection in dental and medical devices. Nat. Med. 1:956-8.

Rideal S, Walker J T A (1903).The standardisation of disinfectants" J. R. Sanit. Inst .24 : 424–441.

Andrews J M (2001). Determination of minimum inhibitory concentrations".Journal of Antimicrobial Chemotherapy. 48: 5–16.

Singleton P (1999). Bacteria in Biology,Biotechnology and Medicine (5th ed.). Wiley. 444–454.

Ashley KL (1983). The antibacterial properties of two commonly used Mouth washes Corsodyl and oraldine Journal of Applied Bacteriology. 56:221–225

Bacanova (2008). Growth rate of test microorganisms. Available at: http://www.ifr.ac.uk/bacanova/project_backg.htm