Antiplasmodial Activity of Combined Bark Extracts of Vernonia amygdalina, Mangifera indica, and Carica papaya at a 1:1:1 Ratio in a Murine Malaria Model
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Abstract
Background: Malaria remains a significant global health burden, exacerbated by the rising prevalence of drug-resistant Plasmodium strains. This critical public health challenge highlights an urgent need for novel anti-malarial therapies, with traditional herbal medicine offering a promising avenue for drug discovery. This study evaluated the antiplasmodial efficacy of bark extracts from Vernonia amygdalina (BB), Mangifera indica (MB), and Carica papaya (PB), investigating both individual treatments and polyherbal combinations in an experimental parasitic infection model.
Methods: Parasitaemia was established in mice, followed by a 5-day curative treatment initiated on day 4 post-infection. Mice were administered 400 mg/kg of each extract, 10 mL of distilled water (negative control), or 5 mg/kg of Chloroquine (CQ) as a positive control. Parasite clearance was assessed on day 9 post-infection.
Results: Our findings demonstrated synergistic antiplasmodial activity in the polyherbal combinations for both aqueous and ethanol extracts, outperforming individual extract treatments. Notably, the triple ethanol combination (BB+MB+PB) exhibited the highest parasite clearance at 89.71%, closely approaching the standard drug CQ (97.89%), representing only an 8.18% difference in efficacy.
Conclusion: These results offer significant insights into the therapeutic potential of these plant extracts, particularly when combined, advocating for further dose-response investigations into their collective anti-malarial properties.
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References
Asangha EE, Igile GO, Iwara IA, Ebong PE, Eseyin OA. Hematological indices of Plasmodium berghei infected mice treated with ethanol extract and fractions of Nauclea latifolia roots. Int J Curr Microbiol Appl Sci. 2017;6(12):2546–56.
Siti F, Farida V, Nuari YR, Viviandhari D, Pertiwi DV. Investigating the impact of surfactant and cosolvent on the polyphenolic content in Arumanis mango leaf extract (Mangifera indica L.). J Sains Farmasi Klinis. 2024;11:39–47.
World Health Organization. Basic malaria microscopy: part 1, learner’s guide. 2nd ed. Geneva: World Health Organization; 2010.
Adetutu A, Olorunnisola OS, Owoade OA, Adesina BT. Antiplasmodial and hepatoprotective potentials of certain traditional antimalarial remedies used in Nigeria. Nat Sci. 2016;14(9):76–86.
Azwanida NN. A review on the extraction methods use in medicinal plants, pertaining to the polarities of solvents. J Tradit Complement Med. 2015;5(2):209–16.
Nawaz H, Shad MA, Rehman N, Andaleeb H, Ullah N. Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Braz J Pharm Sci. 2020;56:e17129.
Uzor PF. Alkaloids from plants with antimalarial activity: a review of recent studies. Evid Based Complement Alternat Med. 2020;2020:8749083.
Musa MA, Ibrahim TA, Tiamiyu HT, Musa MA, Bello A. Antimalarial and antioxidant properties of Vernonia amygdalina (bitter leaf) ethanol leaf extract in Plasmodium berghei-infected mice. J Med Plants Stud. 2024;12(6):33–7.
Abosi AO, Raseroka BH. In vivo antiplasmodial activity of Vernonia amygdalina. Br J Biomed Sci. 2003;60(2):89–91.
Degu A, Mamo H, Tegegne F, Fekadu N. Vernonia amygdalina: a comprehensive review of its traditional uses, phytochemicals, and pharmacological activities. Front Nat Prod. 2024:1347855.
Okpe O, Agada EE, Okoduwa SIR, Upev VA, Abu SM, Adakole O, et al. Aqueous stem bark extract of Mangifera indica suppresses parasitemia and ameliorates anaemia in a mice model of malarial infection. Malays J Biochem Mol Biol. 2021;3(12):105–12.
Yakubu OB, Fodeke H, Abdullahi MM. In vivo evaluation of the antiplasmodial efficacy of Mangifera indica leaf extract in Plasmodium berghei-infected mice. FUDMA J Sci. 2024;8(6).
Babalola BA, Akinwande AI, Otunba AA, Adebami GE, Babalola O, Nwufo C. Therapeutic benefits of Carica papaya: a review on its pharmacological activities and characterization of papain. Arab J Chem. 2024;17(1):105369.
Teng WC, Chan W, Suwanarusk R, Ong A, Ho HK, Russell B, et al. In vitro antimalarial evaluations and cytotoxicity investigations of Carica papaya leaves and carpaine. Nat Prod Commun. 2019;14(1):1934578X1901400110.
Singh SP, Kumar S, Mathan SV, Tomar MS, Singh RK, Verma PK, et al. Therapeutic application of Carica papaya leaf extract in the management of human diseases. Daru. 2020;28(2):735–44.
Alaribe SC, Oladipupo AR, Uche GC, Onumba MU, Ota D, Awodele O, et al. Suppressive, curative, and prophylactic potentials of an antimalarial polyherbal mixture and its individual components in Plasmodium berghei-infected mice. J Ethnopharmacol. 2021;277:114105.
Mhaske S, Borde A, Sitaphale GR, Tathe PR. Combination of herbal formulations: a comprehensive review. Int J Pharm Sci. 2025;3(6):3028–32.
Erhirhie EO, Ikegbune C, Okeke O, Onwuzuligbo CC, Madubuogwu NU, Chukwudulue UM, et al. Antimalarial herbal drugs: a review of their interactions with conventional antimalarial drugs. Clin Phytosci. 2021;7:4.
Ocan M, Loyce N, Ojiambo KO, Kinengyere AA, Apunyo R, Obuku EA. Efficacy of antimalarial herbal medicines used by communities in malaria-affected regions globally: a protocol for systematic review and evidence and gap map. BMJ Open. 2023;13(7):e069771.