Antibacterial Effect of Methanolic Extract of the Root of Aspilia africana

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E. C. Johnson
O. A. Eseyin
A. E. Udobre
P. Ike

Résumé

The leaf of Aspilia Africana (Pers) C.D. Adams (Asteraceae) is widely used in ethno medicinal practices in Tropical Africa because of its ability to stop bleeding and promote rapid healing of wounds. It is also used in the management of problems related to cardiovascular diseases, lumbago, venereal diseases and parasitic infections. This study was carried out on the root part (which has not been very well exploited in ethno medicinal practices) to determine if it is as potent as the leaf part. The methanolic extract of the root was subjected to preliminary phytochemical screening and it has indicated the presence of saponins, tannins cardiac glycosides, flavonoids, terpenoids and carbohydrates. Acute toxicity test showed that it has LD50 of 707.11mg/kg in mice. The in-vitro antibacterial test using agar well diffusion method showed activity against known wound pathogens such as Staphylococcus auerus (clinical isolate), Staphylococcus aureus (ATCC 25922), Staphylococcus aureus (NCTC 8853), Bacillus substiles, Escherichia coli (ATCC 25922), Escherichia coli (NCTC 10418) and Pseudomonas aeruginosa (ATCC 25922). This indicates that the methanolic extract of the root has potentials for use as antibacterial agent in wound care.

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Johnson, E. C. ., Eseyin, O. A. ., Udobre, A. E., & Ike, P. (2011). Antibacterial Effect of Methanolic Extract of the Root of Aspilia africana. Nigerian Journal of Pharmaceutical and Applied Science Research, 1(1), 44–50. Consulté à l’adresse https://nijophasr.net/index.php/nijophasr/article/view/41
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E. C. Johnson

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo

O. A. Eseyin

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo

A. E. Udobre

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo

P. Ike

Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo

Références

Adeniyi, B.A. and R. Odufowora, (2000) In vitro Antimicrobial Properties of Aspilia africana. Afr. J. Biomed. Res., 3(3): 167-70.

Akabue, P. I., Mittal, G. C. and Aguma C. N. (1983) Preliminary Pharmacological Study of some Nigerian Plants; J. Ethnopharmacol. pp 853 – 863.

Anibijuwon, I. I., Dulilemi O. P. and Onifade A. K. (2010) Antimicrobial activity of leaf of Aspilia africana on source pathogen organisms of clinical origin; Ethnobotanical Leaflets 14; pp 390 – 401

Bradshaw, J. L. (1986) Bacterial Identification: Laboratory Microbiology; CBS,

Delhi. Burkill, H.M., (1985) The Useful Plants of West Tropical Africa. Royal Botanic.Gardens, KEW. Vol. 1, 2nd Edn., Families A-D, pp: 446-447.

Dalziel, J. M. (1937) Useful Plants of West Tropical Africa, Crown Agents for Overseas Government and Administration 4 Mill Bank, London, p. 43 Del-Rio, A.

Obdululio B. G., Casfillio, J., Marian F. G., Ortuno, A., (1997), Uses and Properties of Citrus Flavonoids in Okwu, D.E. and Josiah, C. (2006). Evaluation of the Chemical composition of two Nigerian medicinal plants. Afr. J. Biotechnol., 5: 357-361.

El-Tantaway. M. E., Shakhawy, F. S., El-Sohly, M. A., Ross, S. A. (1999), Chemical Composition and Biological Activity of the Essential Oil of the Fruit of Taxodium distichum L. Rich – growing in Egypt in Eweka, A. O., and Eweka A. B. Anti-Ulcer Effect of Aspilia africana Leaf Extract on induced Duodenal Ulcer of Adult Wister Rats (Rattus norvegicus). A Histological study. The Int. J. Alt. Med. 8(1)

Harbourne, J.B., (1984) Phytochemical Methods-A Guide to Modern Technique of Plant Analysis. 2nd Edn., Chapman and Hall, New York, pp: 120.

Hutchinson, J. (1962) Flora of West Tropical Africa, Crown Agents for Overseas

Government and Administration 4 Mill Bank, London, p. 119.

Lorke, D., (1983) A New Approach to Practical Acute Toxicity Testing. Arch. Toxicol., 54: 251187.

Lovian, V. (1980) Antibiotics in Laboratory Medicine, Baltimore, Williams and Williams Okoli, C.O., P. Akah and A. Okoli, 2007. Potential of leaves of Aspilia africana (Compositae) in wound care: an experimental evaluation. BMC Compl. Alter. Med., 7(24) doi: 10.1186/1472-6882-7-24

Okwu, D. E. and Ekeke, O (2003) Phytochemical Screening and Mineral Composition of Chewing Sticks in South Eastern Nigeria. Global J. Appl. Scs. 9: p235 – 238

Okwu, D.E. and Josiah, C. (2006). Evaluation of the chemical composition of two Nigerian Medicinal plants. Afr. J. Biotechnol., 5: 357-361.

Okwuonu, C.U., K.A. Oluyemi, G.D. Baxter, O.A. Adesanya, V.O. Ukwenya, B.I. Odion and D.A. Ofusori, (2008). Effects of Methanolic Extract of Aspilia africana leaf on the Ovarian tissues and weights of wistar rats. Internet J. Alternative Med. 5(2)

Oyewale, A. O., Audu, O. T. and Amupitan, J. O. (2004) A Survey of the Chemical Constituents and Biological Activities of some Medicinal Plants, ChemClass J. pp. 162 – 165.

Saleh, N., Miller, N. J., Parayanga, G., Tijburg, L., Bolwell, G. P., Rice, E., Evans, C. (1995) in Okwu and Josiah,(eds) (2006). Evaluation of the chemical composition of two Nigerian medicinal plants. Afr. J. Biotechnol., 357-361. 5: Sofowora, A. (1993) Medicinal Plants and Traditional Medicine in West Africa, John Wiley and Sons Ltd. New York. P. 109.

Sofowora, E. A. (1982) Medicinal Plants and Traditional Medicine in Africa, Spectrum Books Ltd,

John Wiley Trease, G. E. and Evans, W. C. (2009) Trease and Evans Pharmacognosy, 15th edn. Bailliere, Tindall, London. p. 135 –149; 430.

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