Screening of Hepatoprotective Effects of Four Different Brands of Polyherbal Bitter Formulations against Tetrachloromethane (CCl4) Induced Hepatotoxicity In Wistar Rats
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Abstract
Polyherbal bitters are a diverse group of formulation products used to strenghten and improve the whole digestive system in the body as well as nervous system. The aim of this study is to evaluate the phytochemical constituents and the hepatoprotective activity against tetrachloromethane (CCl4) induced liver injury in wistar rats for four different brands of polyherbal bitter formulations.The phytochemical tests were carried out with standard procedures while thirty (30) rats were used for hepaprotective study with weights ranging from 180 – 260g. The animals were divided into six groups, group 1 served as control and received 0.9% normal saline, group 2 – 6 received CCl4 intraperitoneally on alternate days (five days) for a peroid of ten days , while group 2 – 5 were administered (2.6 ml/kg/day) of each of the four different brands of polyherbal bitter formulations (SAMPLES Y, S, L, B) respectively daily for nine days. The animals were anaesthesized after the last dose of carbon tetrachloride and blood samples were taken via cardiac puncture for hematological and biochemical analysis after which the animals were euthanized and four organs (liver, kidney, lungs and brain) were removed for histological analysis. The CCl4 toxicity in group 6 significantly increased Alanine Aminotransferase (ALT) Aspartate Aminotransferase (AST) and Alkaline Phosphatase (ALP) (39.10 ± 2.4, 131.4 ± 0.73, 208.60 ± 4.6 respectively as compared to normal control group 1 of ALT, AST and ALP, 30.36 ± 1.24, 3.96 ± 1.27, 24.04 ± 3.81 respectively, (P<0.05). It was observed that there was a decrease in enzyme biomarkers (Aspartate and Alanine Transaminase) of liver injury in the herbal mixture treated groups. In the microscopic studies, CCl4 induced toxicity showed haemorrhages, fatty changes and necrosis. All the four herbal formulations conclusively showed marked beneficial effects as revealed in biochemical and histological parameters. It can be concluded that the four polyherbal bitters protect the liver. The present findings demonstrated the efficacy of polyherbal liquid formulations in protecting the liver in CCl4 induced hepatotoxicity in Wistar rats.
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References
Ashok Shenoy, K., S.N. Somayaji and K.L. Bairy, (2001) Hepatoprotective effects of Ginkgo biloba against carbon tetrachloride induced hepatic injury in rats. Indian J. Pharmacol., 33, 260-266.
Babu, B.H., B.S. Shylesh and J. Padikkala,( 2001). Antiioxidant and hepatoprotective effect of Acanthus ilicifolius. Fitoterapia, 72, 272-277.
Dwivedi. Y., R. Rastogi, R. Chander, S.K. Sharma, N.K. Kapoor, N.K. Garg and B.N. Dhawan: (1990) Hepatoprotective activity of picroliv against carbon tetrachloride induced liver damage in rats. Indian J. Med. Res., 92, 195-200.
Drury, RAB.,Wallington,E.A., Cameron, R.(1976).Carliton histological techniques.4th.ed. Oxford University Press, NY. U.S.A . pp279-280
Eisenberg DM, Kessler RC, Van Rompay MI, Kaptchuk TJ, Wilkey SA, Appel S, Davis RB. Perceptions about complementary therapies relative to conventional therapies among adults who use both: results from a national survey. Ann Intern Med 2001;135(5):344-351.
Ellis, G.,Goldberg, D.M.,Spooner, R.J.,(1978). Serum enzyme test in diseases of liver and biliary tree. Am. J. Clin. Pathol., 70: 248-258.
Faulkner, W.R. and J.W. King (1976): Renal Function. Fundamentals in Clinical Chemistry (Ed: N. Teitz). W.B. Saunders, Philadelphia, USA. pp. 975-1014.
Giannini,G., Testa, R. and Savarina, V.,(2005). Liver enzyme alteration: A guide for clinicians. Can. Med. Assoc. J, 172 : 1-7.
Gopinath, C. and Ford E.J.H.: (1975). The role of microsomal hydroxylases in the modification of chloroform hepatotoxicity in rats. Br. J. Exp. Pathol., 56, 412-422.
Harborne JB (1973). Phytochenical methods, Chapman and Hall Ltd; London, 89 – 120
Hawk C, Ndetan H, Evans MW, Jr. Potential role of complementary and alternative health care providers in chronic disease prevention and health promotion: an analysis of National Health Interview Survey data. Prev Med 2012;54(1):18-22.
Kataria, M. and Singh L.N.: (1997). Hepatoprotective effect of Liv-52 and kumaryasava on carbon tetrachloride induced hepatic damage in rats. Indian J. Exp. Biol., 35, 655-657.
Korsrud, G.O. and H.C. Grice (1972): Sensitivity of several serum enzymes in detecting carbon tetrachloride induced liver damage in rats. Toxicol. App. Pharmacol., 22,474-483.
Lai Sam Aru K., Murthy Balakrishma P. and Pillai Sadasivan K., (2007). Screening of hepatotoxicity in Swiss albino mice. J. of Envir. Biol.28(2), 201-207.
Miura, K. Goldstein ., R.S and Morgan D.G.: (1987). Age related differences in susceptibility to renal ischemia in rats. Toxicol. Appl. Pharmacol., 87, 284-296.
McLean, E.K., A.E.M McLean and P.M. Sutton (1969). Perez: Instant Cirrhosis. An improved method of producing cirrhosis of the liver in rats by simultaneous administration of carbon tetrachloride and phenobarbitone. Br. J. Exp.Pathol., 50, 502-506.
Perez-Tomayo, P.: (1983). Is cirrhosis of the liver experimentally produced by CCl4 an adequate model of human cirrhosis? Gastroenterology, 3, 112-120.
Reichling, J.J. and Kaplan, M.M., (1988). Clinical use of serum enzymes in liver diseases.Dg. Dis. Sci. 33: 1601-1614.
Singh, V.K., C.X. George, K.P. Gupta and B.M. (1991). Gupta: Antiviral activity of plant extract Liv 52 in mice experimentally infected with Semliki Forest Encephatitisvirus. Science and Cult ure, 49, 354.
Timbrell, J.A. (1991). Principles of biochemical toxicology. Taylor and Francis, London. pp. 297-312.
Zhao, Z.S. and ‘Brien P.J. O (1996): The prevention of CCl4 induced liver necrosis in mice by naturally occurring methylenedioxybenzenes. Toxicol.Appl. Pharmacol., 140, 411-421.