Extraction, characterization and evaluation of natural chitosan from Penaeus monodon for the formulation of domperidone microspheres
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Résumé
Background: This research work was aimed at extracting and characterizing chitosan obtained from shells of Penaeus monodon for preparation of domperidone sustained release microspheres.
Methods: Extraction took three consecutive steps: deproteination, demineralization and deacetylation. Structure of chitosan, degree of crystallinity, functional groups in chitosan were done through scanning electron microscope, diffractometer, and infrared spectrophotometry respectively. X-ray fluorescence was used to show qualitative and quantitative elemental contents. Degree of deacetylation was carried out using potentiometric titration. Microspheres were prepared by external gelation technique using tripolyphosphate as cross-linking agent and characterized for drug entrapment efficiency and drug release profile. Domperidone release data were analysed according to zero-order kinetics, first order kinetics and Higuchi model.
Results: FTIR spectrum of chitosan contained 12 major peaks between 861.0 and 3276.3 cm-1; Particle size and shape were even at low magnification and a rougher particle surface with increased magnification. XRD showed strong reflections at 2? angles of 15.2°, 16.0°, 17.2°, 23.5°, 38.2° and 50.8. X-ray fluorescence showed heavy presence of calcium having a 14 % of the constituents. Domperidone microspheres loaded chitosan showed the particle size ranging from 50 µm to 179 µm. Degree of deacetylation was 65.2 %. Drug entrapment efficiency was in the range of 39.0 - 78.4 % and about 71.0 % of the drug was released in 41/2 hr. Batches 1-11 followed zero order, via Fickian diffusion.
Conclusion: Chitosan obtained from Penaeus monodon could be used in the formulation of domperidone microspheres with varying natural polymer concentrations.
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Références
Divya. K., Sharrel Robello, Jisha M.S. (2014). A simple and effective method for extraction of high purity chitosan from shrimp waste. International Conference on Advances In Applied Science and Environmental Engineering - ASEE, January 2014. Kualalumpur, Malyasia
Majekodunmi, S.O., Olorunshola E.O., Ofiwe U.C., Udobre A.S and Akpan Ekefre. (2017). Material properties of chitosan from shells of Penaeus monodon. Drug delivery considerations; Journal of coastal life medicine; 5(7): pp, 321-324.
Reddymasu S.C., Soykan I., McCallum R.W. (2007). Domperidone: Review of pharmacology and clinical applications in Gastroenterology. The American Journal of Gastroenterology. 102(9): 2036-2045
Taylor and Francis (2000). Index Nominum 2000: International Drug Directory, pp 366.
Barone, J.A. (1999). Domperidone: a peripherally acting dopamine2-receptor antagonist. The annals of pharmacotherapy. 33(4): 429-40.
Burrows A, Dessart L, LIvne E, Ott C D, Murphy J (2007) Simulations of Magnetically Driven Supernova and Hypernova Explosions in the Context of Rapid Rotation, The Astrophysical Journal, Vol 664, 1.
Olorunsola, E., O., Olaleye, O. J., Majekodunmi, S. O. and Unyime, B. (2015). Extraction and physicochemical characterization of a potential multifunctional pharma-excipient from crab shell wastes. African Journal of Biotechnology, 14(40): 2856-2861.
Wang QZ, Chen XG, Liu N, Wang SX, Liu CS, Meng XH, Liu CG (2006). Protonation constant of chitosan with different molecular weight and degree of deacetylation. Carbohydr. Polym. 65:194-201
De Alvarenga (2011). Characterization and properties of chitosan. Biotechnology of biopolymers. Intech Rijeka Croatia 91-909.
Sonia, T. and Sharma, C. (2011). Chitosan and Its Derivatives for Drug Delivery Perspective. Advances in Polymer Science, pp.23-53.
Yin L, Ding J, He C, Cui L, Tang C and Yin C: Drug permeability and mucoadhesive properties of thiolated chitosan nanoparticles in oral insulin delivery. Biomaterial 2009; 30: 5691 – 5700.
Kumar, K. M. (2000). A review of chitin and chitosan applications. Reactive and Functional Polymers, 46(1), pp.1-27.
Namdev, N. Formulation and evaluation of egg albumin based controlled microspheres of metronidazole. International Journal of Current Pharmaceutical Research. 2016; Vol 8
Artifin, D. Y., Lee, L. Y. & Wang, C. H. Mathematical modeling and simulation of drug release from microspheres: implication to drug delivery systems. Adv Drug Deliv Rev. 2006, 58: 1274-1325.
Desai, K.G.H and Jin, Park H. (2005) Recent Developments in Microencapsulation of Food Ingredients. Drying Technology, 23, 1361-1394.
Denkbas, E. B (2002), Human serum albumin (HAS) adsorption with chitosan microspheres, J appl polym Sci.