Association Between Lifestyle and Genotoxicity in Undergraduate Subjects in Benin City, Nigeria

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Efosa B. Odigie
Olague Iziegbe
Braimah E. Deborah

Résumé

Background of Study: Genotoxicity describes the characteristic of chemical substances known as


genotoxins that harm the genetic material inside a cell, resulting in mutations that may predispose to


cancer.


Methods: This research utilized a cross sectional study design aimed at determining possible association


between lifestyle factors and genotoxicity of undergraduate subjects in Benin City. One hundred and


ninety five (195) subjects, involving one hundred and fifty (150) undergraduates as study subjects and


forty-five (45) intending students as control participated. Questionnaires were used for data collection


while a wooden spatula was used to obtain buccal epithelial cells for micronuclei investigation. The


presence of micronuclei (MN) was assessed under light microscopy and a total of 200 cells per sample


were scored.


Results: The results showed that there was no significant statistical difference (p>0.05) between the mean


micronuclei (Mni) cells counts of the non-cigarette smokers and former smokers; However, the average


Mni count for Shisha smokers was statistically significantly lower than non-shisha smokers. The average


Mni count between alcohol consumers and non-consumers was not statistically significant. The mean


micronuclei (Mni) count per 200 cells was (5.56±1.30) in undergraduate compared to intending students


(3.72±1.39), the mean Mni in age group ? 20yrs was 4.32±1.39 while the average Mni for ? 20yrs of age


was (2.82±1.29) with each of these groups being statistically significant (p-values =0.02).


Conclusion: There is a clear correlation between lifestyle factors and genotoxicity in undergraduate


subjects in this study as observed from the increase in buccal cell micronucleus.

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B. Odigie, E., Iziegbe, O., & E. Deborah, B. (2023). Association Between Lifestyle and Genotoxicity in Undergraduate Subjects in Benin City, Nigeria. Nigerian Journal of Pharmaceutical and Applied Science Research, 12(1), 10–18. https://doi.org/10.60787/nijophasr-v12-i1-501
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