Evaluation of Antioxidant Activity And Chemical Analysis Of The Leaf Of <em>Telfairia occidentalis</em>
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Résumé
The methanol extract of Telfairia occidentalis leaf and n-hexane, ethyl acetate, butanol fractions were evaluated for their free radical scavenging activity, with DPPH assay. n-hexane fraction had the highest activity exhibiting an IC50 of 78.50?g/ml, comparable to that of the commercial antioxidant BHT. Chromatographic analysis showed the presence of three components in n-hexane fraction (2H1, 2H3 and 2H4) with Rf values of 0.86, 0.71 and 0.55, respectively. Two components were seperated in ethyl acetate fraction (2E1 and 2E5) with Rf values of 0.85 and 0.25, respectively, while the three components of butanol fraction (2B1, 2B2, and 2B3) have Rf values of 0.90, 0.88 and 0.83 respectively. In addition, total phenolic content of T. occidentialis was determined as catechin equivalents. The n-hexane fraction which had the highest DPPH free radical scavenging activity also had the highest total flavonoid contents. The high flavonoid content was responsible for the antioxidant and free radical scavenging activities of Telfairia occidentalis leaf.
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