Kinetics and Mechanisms of Doxorubicin Release from hydroxyapatite-sodium alginate nanocomposite

Résumé

In-vitro kinetics and mechanistic study helps the formulation and research scientists to forecast possible rate and mechanism of in-vivo drug release. This study investigates the kinetics and mechanism of Doxorubicin (DOX) (an anticancer drug) release from hydroxyapatite-sodium alginate nanocomposites (HASA). In-situ preparation of hydroxyapatite (HA) in the presence of sodium alginate (SA) was done by wet chemical precipitation method. Drug loading was carried out at neutral pH, while in vitro drug release study was carried out in synthetic body fluid (SBF) at pH 7.4 and 37 0C. The release experimental data was fitted into model-dependent and model independent methods using DDSolver software, an excel add-in. The release half time (t50) increased with increase in SA content. Except for HA and HASA-1%wt, the release of DOX from other formulations can best be explained by first order kinetics. The value of n exponent in Korsmeyer-Peppas model ranged from 0.220 to 0.497, which indicate that DOX release from all the formulations followed Fickian diffusion mechanism. The results of profile comparison indicate that the following release profiles are similar: HASA-5%wt and HASA-20%wt, HASA-20%wt and HASA-33%wt, HASA-33%wt and HASA-50%wt. Addition of SA to HA prolonged the release of DOX and also influenced the kinetics and the mechanism of DOX release from the nanocomposite.