Plasmid profile of multidrug resistant Acinetobacter baumannii strains from wounds of patients attending Federal Medical Centre, Abeokuta, Southwest, Nigeria

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Oluwagbemisola C Fasuyi
Williams E. Ike
David A. Ojo
Akinloye O. Adeboye

Résumé

Acinetobacter baumannii which was once considered non-pathogenic Gram-negative bacteria has emerged as a stubborn nosocomial pathogen. The study was conducted to investigate prevalence, antimicrobial resistance and profile plasmids carried by Acinetobacter baumannii from wounds of patients attending Federal Medical Centre, Abeokuta, Nigeria. Wound swabs of 140 patients were collected from the Bones and Plastic Surgery Department from November 2018 to March 2019. Swabs collected were cultured on Leeds Acinetobacter media. Isolates were identified by biochemical method using Oxoid Microbact™ Gram-negative identification system. Polymerase chain reaction was used to investigate the presence of bla-OXA-51-like gene intrinsic to A. baumannii in isolates observed for authentication. Antimicrobial susceptibility test was performed by the agar disc diffusion method and inhibition zones were interpreted following Clinical and Laboratory Standard Institute guidelines. Isolates were cured using Sodium Dodecyl Sulphate and subjected to second antimicrobial susceptibility test. A total of 18 (12.8%) isolates were identified as Acinetobacter baumannii from 140 patients. All the isolates (100%) were resistant to at least three antimicrobial agents. Cured A. baumannii isolates showed that 11 (61%) of the multi-drug resistant isolates were plasmid-mediated, with plasmid sizes ranging from 60-1,333 bp. This study revealed high rate of resistance to multiplicity of antimicrobial agents by plasmids carried on strains of Acinetobacter bauamannii.

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Fasuyi , O. C., Ike , W. E., Ojo , D. A., & Adeboye, A. O. (2020). Plasmid profile of multidrug resistant Acinetobacter baumannii strains from wounds of patients attending Federal Medical Centre, Abeokuta, Southwest, Nigeria. Nigerian Journal of Pharmaceutical and Applied Science Research, 9(2), 47–51. Consulté à l’adresse https://nijophasr.net/index.php/nijophasr/article/view/343
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Bibliographies de l'auteur-e

Oluwagbemisola C Fasuyi

Department of Microbiology, College of Biological Sciences, Federal University of Agriculture Abeokuta, Ogun State Nigeria.

Williams E. Ike

Department of Microbiology, College of Biological Sciences, Federal University of Agriculture Abeokuta, Ogun State Nigeria.

David A. Ojo

Department of Microbiology, College of Biological Sciences, Federal University of Agriculture Abeokuta, Ogun State Nigeria.

Akinloye O. Adeboye

Department of Biochemistry, College of Biological Sciences, Federal University of Agriculture Abeokuta, Ogun State Nigeria

Références

Chopade BA, Wise PJ, Towner KJ. (1985). Plasmid transfer and behaviour in Acinetobacter calcoaceticus EBF65/65. Journal of General Microbiology. 131: 2805 -2811.

Clinical and Laboratory Standard Institute (2019). M100 Performance Standards for Antimicrobial Susceptibility Testing; A CLSI supplement for global application. Clinical and Laboratory Standard Institute, 29th Edition, Vol. 39 (No 1).

Demirdal T, Sari US, Nemli SA. (2016). Is inhaled colistin beneficial in ventilator associated pneumonia or nosocomial pneumonia caused by Acinetobacter baumannii? Annals of Clinical Microbiology and Antimicrobials. 15(1):1-6.

Hadis D, Majid B, Amir E. (2015). Plasmid Curing Assay in Clinical Isolates of Antibiotic Resistant Acinetobacter baumannii. Microbiology Journal. 5 (2): 43-48.

Ike WE, Adeniyi BA, Soge OO. (2014). Prevalence of Multidrug Resistant Acinetobacter baumannii in Eight Tertiary Hospitals in Southwestern Nigeria. New York Science Journal; 7(11):86-93]. (ISSN: 1554-0200).

Iregbu KC, Ogunsola FT, Odugbemi TO. (2002). Infections caused by Acinetobacter species and their susceptibility to 14 antibiotics in Lagos University Teaching Hospital, Lagos. West African Journal of Medicine. 21: 226 - 229.

Lopes BS, Gould IM, Opazo AF, Amyes SGB. (2012). The resistant profile of Acinetobacte rbaumannii strains isolated from Aberdeen royal infirmary. International Journal of Antimicrobial agent. 12, 004.

Montefour K, Frieden J, Hurst S, Helmich C, Headley D, Martin M, Boyle DA. (2008): Acinetobacter baumannii: An emerging multidrug resistant pathogen in critical care. Critical Care Nurse. 28: 15 – 25.

Muhammad A, Iqbal AA, Shafiq UR. (2018). Insight into Acinetobacter baumannii: pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infection and Drug Resistance. 11: 1249 - 1260.

Odewale G, Adefioye GO, Ojo J, Adewumi FA, Olowe OA. (2016). Multidrug Resistance of Acinetobacter baumannii In Ladoke Akintola University Teaching Hospital, Osogbo, Nigeria. European Journal of Microbiology and Immunology. 6 (3): 238–243.

Potron A, Poirel L, Nordmann P. (2015). Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii mechanisms and epidemiology. International Journal of Antimicrobial Agents. 45:568–585.

Ruiqiang Xie, Xiaohua D, Zhang QZ, Bo Peng, Jun Z. (2018). Analysis of global prevalence of antibiotic resistance in Acinetobacter baumannii infections disclosed a faster increase in OECD countries. Emergence of Microbes Infection. 7:31.

World Health Organization, 2017. Drug-resistant bacteria ranked. Macmillan publishers limited part of Springer Nature; Vol 543.