Molecular basis of Carbapenem-Resistant Acinetobacter baumannii from Southwest, Nigeria
Barre latérale de l'article
Contenu principal de l'article
Résumé
Information on the molecular basis of resistance to carbapenem resistant Acinetobacter baumannii (CRAB) in Nigeria is sparse. This study was aimed at charaterising the molecular basis of CRAB isolated from tertiary hospitals in Southwest Nigeria. Identity of clinical isolates was confirmed using Oxoid 12E MicrobactTM Gram-negative identification system, blaOXA-51-like primers, and 16S-rRNA sequencing. Antimicrobial susceptibility was performed by disk diffusion method using 34 antimicrobial agents belonging to 8 classes of antibiotics. Molecular characterization was conducted on 21 isolates with resistance phenotype to imipenem and meropenem. Detection and characterization of carbapenem resistance determinants were achieved by PCR. Reference strains, A. baumannii type strain ATCC 17978 and Acinetobacter baylyi ADP1 were used as controls. All isolates were resistant to ?14 antimicrobial agents tested, with 95.9% isolates resistant to 20-34 antibiotics. Resistant phenotype of 78.7% and 57.4% were recorded for meropenem and imipenem respectively, with ciprofloxacin (36.1%), amikacin (37.9%) and polymyxin B (39.3%). Oxacillinase gene, blaOXA-51-like was detected in all isolates, and blaOXA-23 carrying ISAba1 element upstream of gene within genomic resistant island (GRI) TnAbaR1 and cephalosporinase gene, blaADC-7 were also detected in (18/21) of the isolates. Carbapenem resistant A. baumannii isolates from Southwest Nigeria encode genes that code for resistance to carbapenem.
Téléchargements
Renseignements sur l'article
Cette œuvre est sous licence Creative Commons Attribution - Pas d'Utilisation Commerciale - Pas de Modification 4.0 International.
Références
Al-Agamy, M.H., Khalaf, N.G., Tawfick, M.M., Shibl, A.M., Kholy, A.E. (2014). Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt. Int J Infect Dis, 22:49–54.
Bagheri, Josheghani, S., Moniri, R., Firoozeh, F., Sehat, M., Dasteh, Goli Y. (2015). Susceptibility Pattern and Distribution of Oxacillinases and bla PER-1 Genes among Multidrug Resistant Acinetobacter baumannii in a Teaching Hospital in Iran. J Pathog, 95:7259.
Bahador, A., Raoofian, R., Pourakbari, B., Taheri, M., Hashemizadeh, Z., Hashemi, F.B. (2015). Genotypic and antimicrobial susceptibility of carbapenem-resistant Acinetobacter baumannii: analysis of ISAba elements and blaOXA-23-like genes including a new variant. Front Microbiol, 6:1249.
Cicek, A.C., Saral, A., Iraz, M., Ceylan, A., Duzgun, A.O., Peleg, A.Y., Sandalli, C. (2014). OXA- and GES-type ?-lactamases predominate in extensively drug-resistant Acinetobacter baumannii isolates from a Turkish University Hospital. Clin Microbiol Infect, 20: 410 – 415.
Clinical and Laboratory Standards Institute. (2017). Performance standards for antimicrobial susceptibility testing. 27th ed. Wayne, PA: CLSI, CLSI supplement M100.
Evans, B.A., Amyes, S.G.B. (2014). OXA ?-lactamases. Clin Microbiol Rev, 27(2):241–263.
Fonseca, E.L., Scheidegger, E., Freitas, F.S., et al. (2013). Carbapenem-resistant Acinetobacter baumannii from Brazil: role of carO alleles expression and blaOXA-23 gene. BMC Microbiol, 13:245.
Fournier, P.E., Vallenet, D., Barbe, V., et al. (2006). “Comparative genomics of multidrug resistance in Acinetobacter baumannii,” PLoS Genet, 2:1.
Hamidian, M., Holt, K.E., Hall, R.M. (2015). Genomic resistance island AGI1 carrying a complex class 1 integron in a multiply antibiotic-resistant ST25 Acinetobacter baumannii isolate. J Antimicrob Chemother, 70: 2519-23.
Higgins, P.G., Pérez-Llarena, F.J., Zander, E.A., Fernández, G., et al. (2013). “OXA-235, a novel class D ?-lactamase involved in resistance to carbapenems in Acinetobacter baumannii,” Antimicrob Agents Chemother, 57:2121–2126.
Huang, Z., Li, J., Shui, J., Wang, H., Hu, Y., Zou, M. (2019). Co-existence of blaOXA-23 and blaVIM in carbapenem-resistant Acinetobacter baumannii isolates belonging to global complex 2 in a Chinese teaching hospital. Chin Med J, 132:1762.
Japoni, S., Farshad, S., Ali, A.A., Japoni, A. (2011). Antibacterial susceptibility patterns and cross-resistance of Acinetobacter, isolated from hospitalized patients, Southern Iran. Iran Red Crescent Med J, 13:832–836.
Joshi, P.R., Acharya, M., Kakshapati, T., Leungtongkam, U., Thummeepak, R., Sitthisak, S. (2017). Co-existence of blaOXA-23 and blaNDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance. Antimicrob Resist Infect Control, 6:21.
Kim, S.Y., Jung, J.Y., Kang, Y.A., Lim, J.E., Kim, E.Y., Lee, S.K., et al. (2012). Risk factors for occurrence and 30-day mortality for carbapenem-resistant Acinetobacter baumannii bacteremia in an intensive care unit. Journal of Korean Medical Science, 27:939–947.
Krizova, L., Dijkshoorn, L., Nemec, A. (2011). Diversity and Evolution of AbaR Genomic Resistance Islands in Acinetobacter baumannii Strains of European Clone I. Antimicrob Agents Chemother, 55: 3201-3206.
Kuo, S.C., Lee, Y.T., Yang-Lauderdale, T.L., Huang, W.C., Chuang, M.F., Chen, C.P., Su, S.C., Lee, K.R. and Chen, T.L. (2015). Contribution of Acinetobacter-derived cephalosporinase-30 to sulbactam resistance in Acinetobacter baumannii. Front Microbiol, 6:231.
Lee, H.Y., Chen, C.L., Wu, S.R., Huang, C.W., Chiu, C.H. (2014). Risk factors and outcome analysis of Acinetobacter baumannii complex bacteremia in critical patients. Crit Care Med, 42:1081–1088.
Lee, Y.T., Kuo, S.C., Chiang, M.C., et al. (2011). Emergence of carbapenem-resistant non-baumannii species of Acinetobacter harboring a blaOXA-51-like gene that is intrinsic to A. baumannii. Antimicrob Agents Chemother, 56: 1124–1127.
Lévesque, C., Piche, L., Larose, C., Roy, P.H. (1995). PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother, 39: 185–191.
Magiorakos, A.P., Srinivasan, A., Carey, R.B., Carmeli, Y., Falagas, M.E., Giske, C.G., et al. (2012). Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect, 18:268–81.
Mohajeri, P., Farahani, A., Feizabadi, M.M., Ketabi, H., Abiri, R., Najafi, F. (2013). Antimicrobial susceptibility profiling and genomic diversity of Acinetobacter baumannii isolates: a study in western Iran. Iran J Microbiol, 5:5195–5202.
Moniri, R., Farahani, R.K., Shajari, G., Shirazi, M.H.N., Ghasemi, A. (2010). Molecular epidemiology of aminoglycosides resistance in Acinetobacter spp. with emergence of multidrug-resistant strains. Iran J Public Health, 39:63–68.
Munoz-Price, L.S. and Weinstein, R.A. (2008). “Acinetobacter infection,” N Engl J Med; 358:1214–1281.
Pagano, M., Martins, A.F., Barth, A.L. (2016). Mobile genetic elements related to carbapenem resistance in Acinetobacter baumannii. Braz J Microbiol, 47:785–92.
Post, V., White, P.A., Hall, R.M. (2010). Evolution of AbaR-type genomic resistance islands in multiply antibiotic-resistant Acinetobacter baumannii. J Antimicrob Chemother, 65:1162–1170.
Queenan, A.M., Pillar, C.M., Deane, J., Sahm, D.F., Lynch, A.S., Flamm, R.K., et al. (2012). Multidrug resistance among Acinetobacter spp. in the USA and activity profile of key agents: results from CAPITAL Surveillance 2010. Diagn Microbiol Infect Dis, 73:267–270.
Rahbar, M., Mehrgan, H., Aliakbari, N.H. (2010). Prevalence of antibiotic-resistant Acinetobacter baumannii in a 1000-bed tertiary care hospital in Tehran, Iran. Indian J Pathol Microbiol, 53:290–293.
Rose, A., (2010). TnAbaR1: A novel Tn7-related transposon in Acinetobacter baumannii that contributes to the accumulation and dissemination of large repertoires of resistance genes, Bioscience Horizons: Int J Stud Res; 3:40–48.
Rosser, S.J., Young, H.K. (1999). Identification and characterization of class 1 integrons in bacteria from an aquatic environment. J Antimicrob Chemother, 44:11–18.
Safari, M., Saidijam, M., Bahador, A., Jafari, R., Alikhani, M.Y. (2013). High prevalence of multi-drug resistance and metallo-beta-lactamase (MBL) producing Acinetobacter baumannii isolated from patients in ICU wards, Hamadan. J Res Health Sci, 13:162–167.
Shoja, S., Moosavian, M., Peymani, A., Tabatabaiefar, M.A., Rostami, S., Ebrahimi, N. (2013). Genotyping of carbapenem resistant Acinetobacter baumannii isolated from tracheal tube discharge of hospitalized patients in intensive care units, Ahvaz, Iran. Iran J Microbiol, 5:315–322.
Turton, J.F., Baddal, B., and Perry, C. (2011). Use of the accessory genome for characterization and typing of Acinetobacter baumannii. J Clin Microbiol, 49:1260–1266.
Turton, J.F., Ward, M.E., Woodford, N., Kaufmann, M.E., Pike, R., Livermore, D.M., et al. (2006). The role of ISAba1in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiol Lett, 258:72–7.
Vijayakumar, S.B.A., Kanthan, K., Veeraraghavan, B. (2018). Whole-genome shotgun sequences of seven colistin-resistant Acinetobacter baumannii isolates from bacteraemia. J Glob Antimicrob Resist, 12:155–6.
Yazdansetad, S., Najari, E., Ghaemi, E.A., Javid, N., Hashemi, A., Ardebili, A. (2019). Carbapenem-resistant Acinetobacter baumannii isolates carrying the blaOXA genes with an upstream ISAba1: The first report of a novel OXA subclass from Iran. J Glob Antimicrob Resist, 18: 95-99.
Zhao, Y., Hu, K., Zhang, J., Guo, Y., Fan, X., Wang, Y., Divine, M.S., and Zhang, X. (2019). Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the Eastern Heilongjiang Province, China. BMC Infect Dis, 19:452.