Evaluation of the Effects of Citrus paradisi (Rutaceae) Fruit Juice on Electrolyte, Hepatic, Haematological and Histological Derangements in Streptozotocin-Induced Diabetic rats.
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This study investigated the effects of Citrus paradisi fruit juice on biochemical, haematological and histopathological indices in streptozotocin-induced diabetic rats. Wistar rats of either sex (180-300 g) were fasted overnight and diabetes mellitus induced using streptozotocin 40 mg/kg IP. Diabetes mellitus (fasting blood glucose ? 200 mg/dl) was confirmed 48 hours later. The rats were randomly grouped into six (n = 5). Fourteen days later, the rats were sacrificed under chloroform anaesthesia. Vital organs (kidneys, liver and pancreas) and blood samples were obtained for histopathological, biochemical and haematological analysis. Citrus paradisi (500 mg/kg and 1000 mg/kg) caused significant (p < 0.05) elevations in red blood cell counts and red blood cell indices. Citrus paradisi (250 mg/kg, 500 mg/kg and 1000 mg/kg) caused significant (p < 0.05) reductions in white blood cell and platelet counts but did not significantly affect the differential white blood cell count. Liver transaminases and total bilirubin were reduced significantly (p < 0.05) while the total protein was significantly increased (p < 0.05). Citrus paradisi (500 mg/kg and 1000 mg/kg) brought about significant improvements in the histopathological indices in diabetic rats. These data suggest that Citrus paradisi ameliorates biochemical, haematological and histopathological changes associates with diabetes mellitus.
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Références
Adeneye AA. Hypoglycemic and hypolipidemic effects of methanolseed extract of Citrus paradisi Macfad (Rutaceae) in alloxan-induced diabetic Wistar rats. Niger Q J Hosp Med 2008; 18: 211–215.
Akinmoladun AC and Akinloye O (2007). Prevention of the onset of hyperglycaemia by extracts of Aloe barbadensis in rabbits treated with alloxan. African Journal of Biotechnology; 6 (8):1028-1030.
Alam M, Subhan N, Rahman M, Uddin J, Reza M, Sarker D (2014). Effect of citrus flavonoids, naringin and naringenin, on metabolic syndrome and their mechanisms of action. Adv. Nutr.; 5: 404–417, doi:10.3945/an.113.005603.
Arun, G.S, Ramesh, K.G (2002). Improvement of insulin sensitivity by perindopril in spontaneously hypertensive and streptozotocin diabetic rats. Indian Journal of Pharmacology, 34 :156-164.
Buchner I, Medeiros N, Lacerda D, Normann BM, Gemelli T, Rigon P, Wannmacher CMD, Henriques JAP, Dani C, Funchal C (2014). Hepatoprotective and antioxidant potential of organic and conventional grape juices in rats fed a high-fat diet. Antioxidants 3:323-338
Cavia-Saiz M, Busto MD, Pilar-Izquierdo MC, Ortega N, Perez-Mateos M, Muñiz P (2010). Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: a comparative study. J Sci Food Agric. 90:1238–44.
Chukwuma OO, Chidozie EA, Ikenna IK, Augusta NC and Jeremiah OS (2016). Anti-diabetic and renal protective effect of the fruit juice of Citrus Paradisi on alloxan induced diabetic male albino wistar rats. Der Pharmacia Lettre; 8:32-38
Doumas BT, Watson WA and Briggs HG (1971). Clinica Chimica Acta 31:87
Eleazu CO, Eleazu CK, Chukwuma S and Essien UN (2013). Review of the mechanism of cell death resulting from streptozotocin challenge in experimental animals, its practical use and potential risk to humans. Journal of Diabetes & Metabolic Disorders, 2013; 12:60.
Evans, W.C. Trease and Evans Pharmacognosy (2002) 15th Edition. London: W.B. Sanders.
Fuji, H., Yokozawa, T., Kim, Y.A., Tohga, C and Nonaka, G (2006). Mini Reviews in Medicinal Chemistry, 7: 663-678
Gate SF (2017) What are the Effects of Grapefruit on Diabetes. Healthy Eating. Retrieved October 21, 2018 from https://healthyliving.sfgate.com//effects-grapefruit-diabetes-2067.html
Grant GH (1987), Amino acids and protein: Fundamentals of Clinical Chemistry. Philadelphia USA: WB Sanders Company. Pp 328-329.
Hanley MJ, Cancalon P , Widmer WW, and Greenblatt DJ (2011). The effect of grapefruit juice on drug disposition Expert Opin Drug Metab Toxicol. 7(3): 267–286. doi:10.1517/17425255.2011.553189.
Herbal Medicine, Grapefruit (2000). (http://www.holistic-online.com/Herbal-Med/_Herbs/h_grapefruit.htm).
Hung W, Suh J H and Wang Y (2017). Chemistry and health effects of furanocoumarins in grapefruit. Journal of food and drug analysis 2571e8 3
Ikemura M and Sasaki Y, Giddings JC, Yamamoto J (2012). Preventive effects of hesperidin, glucosyl hesperidin and naringin on hypertension and cerebral thrombosis in stroke-prone spontaneously hypertensive rats. Phytother Res 26:1272–7.
International Diabetes Federation (Brussels) (2012): Diabetes at a glance. Africa (AFR). Available at https://www.idf.org/sites/default/files/IDF_ AFR_5E_Update_FactSheet_0.pdf. Accessed February 13, 2017.
Joo HL, Si HY, Jung MOH and Myung GL (2010): Pharmacokinetics of drugs in rats with diabetes mellitus induced by alloxan or streptozocin: comparison with those in patients with type I diabetes mellitus. J Pharm Pharmacol 62:1–23
Jung UJ, Lee M-K, Jeong K-S and Choi M-S (2004). The hypoglycemic effects of hesperidin and naringin are partly mediated by hepatic glucose-regulating enzymes in C57BL/KsJ-db/db Mice. J Nutr 134:2499–503
Kiani J and Imam SZ (2007): Medicinal importance of grapefruit juice and its interaction with various drugs. Nutrition Journal, 6:33 doi:10.1186/1475-2891-6-33.
Kolanjiappan K, Manoharan S, Kayalvizhi M (2002). Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clin Chim Acta, 326:143-9.
Kotharia, R and Bokariya, PA (2012). Comparative Study of haematological parameters in type 1 diabetes mellitus patients and healthy young adolescents. International Journal of Biological and Medical Research, 2012; 3:2429-2432.
Lee MH, Yoon S and Moon JO (2004). The flavonoid naringenin inhibits dimethylnitrosamine- induced liver damage in rats. Biol Pharm Bull 27: 72–6.
Liamis G, Liberopoulos E, Barkas F and Elisaf M (2014): Diabetes mellitus and electrolyte disorders. World J Clin Cases, 2(10): 488-496
Lorke, D (1983). A new approach to practical acute toxicity testing. Archive Toxicology, 54:275-287.
Magoshes and Vallee (1956). Flame photometry and spectrophotometry and spectrometry. Journal of International Science 2 (1) 13-16
Mahmoud AM (2013). Haematological alterations in diabetic rats - Role of adipocytokines and effects of citrus bioflavonoids. Excli Journal 12: 647-657.
Mahmoud AM and Hussein OE (2016). Anti-Diabetic effect of naringin: Insights into the molecular mechanism. Diabetes Obes Int J 1: 000128.
Maritim AC and Sander RA (2003), Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 17: 24–38.
Martin K, Appel C (2010). Polyphenols as dietary supplements: A double-edged sword. Nutrition and Dietary Supplements 2:1–12
McPherson P, McEneny J (2010). The biochemistry of ketogenesis and its role in weight management, neurological disease and oxidative stress. Journal of Physiology and Biochemistry 68:141– 151.
Mohammed A, Mohammed AI and Shahidul I (2014). African Medicinal Plants. Planta Med 80: 354–377
Murunga AN, Miruka DO, Driver C, Nkomo FS, Cobongela SZZ, and Owira PMO (2016). Grapefruit derived flavonoid naringin improves ketoacidosis and lipid peroxidation in type 1 diabetes rat model. PLoS ONE 11: e0153241.
Neelesh M, Sanjay J and Sapna M (2010). Antidiabetic potential of medicinal plants. Acta Pol Pharm 67: 113–118.
Nzuza S, Zondi S and Owira PMO (2017) Naringin prevents HIV-1 protease inhibitorsinduced metabolic complications in vivo. PLoS ONE 12(11): e0183355. https://doi.org/10.1371/ e0183355
Ohlsson A, Aher SM (2012). Early erythropoietin for preventing red blood cell transfusion in preterm and/or low birth weight infants. Cochrane Database Syst Rev 9:CD004863.
Osigwe, C.C., Akah, P.A. and Nworu, C.S (2017). Biochemical and haematological effects of the leaf extract of Newbouldia laevis in alloxan- induced diabetic rats. Journal of Biosciences and Medicines 5:18-36.
Owira PMO and Ojewole JAO. Grapefruit juice improves glycaemic control but exacerbates metformin-induced lactic acidosis in diabetic rats. Methods Find Exp Clin Pharmacol 2009; 31: 563-570.
Oyedemi, S.O., Adewusi, E.A., Aiyegoro, O.A and Akinpeanolu, D.A (2011). Antidiabetic and hematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on Streptozotocin-induced diabetic wistar rats. Asian Pacific Journal of Tropical Biomedicine 1: 353-358.
Pari L, Amudha K (2011). Hepatoprotective role of naringin on nickel-induced toxicity in male Wistar rats. Eur J Pharmacol. 650:364–70.
Powers AC and D’Alessio D (2011). Endocrine pancreas and pharmacotherapy of diabetes mellitus and hypoglycaemia. In: Brunton LB, Bruce C, B Knollman, eds. Goodman and Gilman’s the pharmacological basis of therapeutics 12th edition, New York, Mc Graw Hill: 1237-1274.
Rana, S., Singh, R and Verma, S. Protective effects of few antioxidants on liver function in rats treated with cadmium and mercury. Indian Journal of Experimental Biology. 1996; 34 :177-179.
Reitman S and Frankel S (1957). American Journal of Clinical Pathology 28:56
Renugadevi J and Prabu SM (2009). Naringenin protects against cadmium-induced oxidative renal dysfunction in rats. Toxicology 256:128–34.
Rheney, C.C and Kirk, K.K (2000). Performance of three blood glucose meters. Annual Pharmacotherapy; 34: 317-321.
Row E, Brown SA, Stachulski AV and Lennard MS (2006). Development of novel furanocoumarin dimmers as potent and selective inhibitors of CYP3A4. Drug Metab Dispos; 34 :324 - 330.
Schales O and Schales SS (1941). A simple and accurate method for the determination of chloride in biological fluids. Journal of Biological Chemistry 140 (5): 879-882
Schmidt E and Schmidt FW (1963). Enzym Biol Clin 3:1
Shah, N.A and Khan, M.R (2014). Antidiabetic effect of Sida cordata in alloxan- induced diabetic rats. BioMed Research International Article ID: 671294.
Sofowora. A (2008). Medicinal Plants and Traditional Medicine in Africa (3rd Ed.) Ibadan, Nigeria: Spectrum Books Ltd. 2008; pp. 199-205.
Srinivasan K and Ramarao P (2007). Animal models in type 2 diabetes research: An overview. Indian J Med Res 125:451-472.
Tietz NW (1995). Clinical Guide to Laboratory Test. Philadelphia: WB Sanders Company. 3rd edition Pp 518-519
Tripoli E, Guardia ML, Giammanco S, Majo DD and Giammanco M (2007). Citrus flavonoids: molecular structure, biological activity and nutritional properties: a review. Food Chem; 104:466–79.
Uko, E.K., Erhabor, O., Isaac, I.Z., Abdulrahaman, Y., Adias, T.C., and Sani, Y (2013), Some haematological parameters in patients with Type 1 Diabetes in Sokoto, North Western Nigeria. Journal of Blood & Lymph 3:2165-7831.
Van Skye DD and Neil FM (1924). The determination of gases in blood and other solutions. Journal of Biological Chemistry 6(6):523.
Whiting DR, Guariguata L, Weil C and Shaw J (2011). IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabet Res Clin Pract; 94: 311–321.
Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, Sole J, Nichols A, Ross JS and Tartaglia LA (2003). Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest 112:1821–30.