Evaluation of the Larvicidal Activity Of The Root Extracts of Allamanda Cathartica L (Apocynaceae) On Aedes Aegypti Larvae

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Okwubie Lambert
Onu Umunakwe

Résumé

Aedes aegypti is a species of mosquito that transmits yellow fever, zika virus fever, dengue fever and chikungunya diseases. Allamanda cathartica L is an evergreen tropical plant that is grown as a climbing vine or pruned as a shrub, belonging to the family Apocynaceae. The aim of this research is to evaluate the larvicidal activity of Allamanda cathartica root on Aedes aegypti larva. The acetone and methanol extracts were obtained by exhaustive soxhlet extraction, while the aqueous extract was obtained through maceration. A stock solution of 10mg/ml was prepared for each extract from which six different concentrations (0.5mg/ml, 1.0mg/ml, 2.0mg/ml, 3.0mg/ml, 4.0mg/ml and 5mg/ml in 100ml) were made and used for the test in accordance with World Health Organization (WHO) guideline. A control was also prepared for each extract by adding 1ml of the solvent to 100ml of water. A total of 25 larvae were used for each of the concentration and mortality was recorded at 24, 48 and 72 hours. The three extracts showed larvicidal activity against Aedes aegypti larva with acetone extract having the highest activity followed by methanol and aqueous extract respectively. At 72 hours, the LC50 of acetone, methanol and aqueous extracts were 0.61mg/ml, 2.98mg/ml and 6.52mg/ml respectively. The result shows that acetone extract of the root of Allamanda cathartica could be a veritable source of Larvicides.

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Lambert, O., & Umunakwe, O. (2020). Evaluation of the Larvicidal Activity Of The Root Extracts of Allamanda Cathartica L (Apocynaceae) On Aedes Aegypti Larvae. Nigerian Journal of Pharmaceutical and Applied Science Research, 6(1), 61–64. Consulté à l’adresse https://nijophasr.net/index.php/nijophasr/article/view/172
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Okwubie Lambert

Department of Pharmacognosy and Phytotherapy, University of Port Harcourt

Onu Umunakwe

Department of Pharmacognosy and Phytotherapy, University of Port Harcourt

Références

Etukudo, I. (2003). Ethnobotany.Conventional and Traditional uses of plants.Vol. 1. TheVerdict Press, Uyo, p. 191.

Finney, D.J. (1971). Probit analysis. Cambridge: Cambridge University Press.

Harborne, J.B. (1984). Phytochemical Methods.Chapman and Hall, London. pp. 166-226.

Iwu, M.M., Jackson, E., Tally, J.D., Klayman, D,I. (1993). Evaluation of plant extracts for antileishmanial activity using a mechanism based radiorespirometricmicrotechnique (Rnm). Planta Medica, 58: 436-441.

Nwabor, Ozioma Forstinus Dibua, Uju Marie-Esther Nnamonu, Emmanuel Ikechukwu Odiachi, Osita Dickson, Dickson

Okoro,Onyekwwere Joseph (2014). Pulp Extracts of Picralima nitida: a Larvicidal Agent in MalariaVector Control. Journal of Biology, Agriculture and Healthcare 4(8): 69 – 73

Roark, R.C. (1947). Some promising insecticidal plants.EconBot ;1: 437-45.

Santos, I. de A.; Souza, F.I.M.A.; Akisue, G.; Coelho, F.A. da S.; Coelho, M.D.G. (2013). Evaluation of the ovicidal and larvicidal activity of ten plant Extracts against Ancylostoma spp. Journal of Revista de patologia tropical 2013,42(2): 209-216

Sofowora, A. (2006). Medicinal Plants and Traditional Medicine in Africa.Spectrum BookLimited, Ibadan, pp. 150 – 183

.

Ubulom, M. E., Imandeh, N. G., Udobi, C.E. and IIya, I. (2012) Larvicidal and Antifungal Properties of Picralima nitida (Apocynaceae) Leaf Extracts. European Journal of Medicinal Plants 2(2): 132-139

WHO (2005).Guidelines for Laboratory and Field Testing of Mosquito larvicides. WHO communicable disease control, prevention and eradication. WHO pesticide evaluation scheme WHO/CDS/WHOPES/GCDPP/2005.13.

WHO (2015). Mosquito borne diseases www.who.int/neglected_diseases/vector_ecology/mosquito-borne-diseases 6th January, 2017

Yang RZ, Tang CS (1988). ,Pant used for pest control in China: a literature review. Journal of Economic Botany. 42(3): 376 - 406