Chemical and antimicrobial evaluations of a culture of Phoma sp., an endophytic fungus isolated from Rhizophora racemosa.

Contenu principal de l'article

Olusegun S. Ajala
Afolabi S. Lamina
Titilope A. Adelekan
Akinsinde K. Adewale

Résumé

Inner tissues of the aerial roots of the mangrove medicinal plant Rhizophora racemosa were subjected to standard fungal isolation techniques, leading to the isolation and subsequent large-batch culturing of an endophytic coelomycetous fungal morphotype, identified as Phoma sp. Extracts of the culture were obtained after a 14-day fermentation period, subjected to chemical evaluation using standard phytochemical methods and antimicrobial evaluation by agar diffusion techniques. Extracts of the fungal culture demonstrated broad spectrum antibacterial activities against Gram positive and Gram negative organisms and were found to be rich in diverse bioactive chemical groups including alkaloids, tannins, saponins and cardiac glycosides. A Phoma sp. is thus, herein, reported as the first from the endophytic community of Rhizophora racemosa and its culture recommended as a veritable lead source for the discovery of new broad spectrum antibacterial drugs and other therapeutic agents.

Téléchargements

Les données relatives au téléchargement ne sont pas encore disponibles.

Renseignements sur l'article

Comment citer
Ajala, O. S. ., Lamina, A. S. ., Adelekan, T. A. ., & Adewale, A. K. . (2020). Chemical and antimicrobial evaluations of a culture of Phoma sp., an endophytic fungus isolated from Rhizophora racemosa. Nigerian Journal of Pharmaceutical and Applied Science Research, 5(1), 7–14. Consulté à l’adresse https://nijophasr.net/index.php/nijophasr/article/view/112
Rubrique
Articles
Bibliographies de l'auteur-e

Olusegun S. Ajala

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, CMUL Campus, PMB 12003, Surulere, Lagos.

Afolabi S. Lamina

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, CMUL Campus, PMB 12003, Surulere, Lagos.

Titilope A. Adelekan

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Lagos, CMUL Campus, PMB 12003, Surulere, Lagos.

Akinsinde K. Adewale

Clinical Science Division, Nigerian Institute of Medical Research, 6,Edmund Crescent, Off Murtala Mohammed way, P.M.B. 2013, Yaba, Lagos.

Références

REFERENCES Aveskamp MM, De Gruyter J, Crous PW (2008). Biology and recent developments in the systematics of Phoma, a complex genus of major quarantine significance. Fungal Diversity 31:1-8

Bacon CW, White Jn. JF (2000). Microbial endophytes. Marcel Dekker, New York; p 487

Bandaranayake WM (2002). Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetlands Ecology and Mnagement 10:421-452

Boerema GH,Gruyter J, Noordeloos ME and Hamers MEC(2004). Phoma identification manual. CABI Publishing, CAB International, Wallingford, Oxfordshire, UK, p. 470

Caruso M, Colombo AL, Fedeli L, Pavesi A, Quaroni S, Saracchi M and Ventrella G (2000). Isolation of endophytic fungi and actinomycetes taxane producers. Annals of microbiology 50(1): 3-14

Dreyfuss MM and Chapela IH (1994). Potential of fungi in the discovery of novel, low-molecular weight pharmaceuticcals. In: Gulls VP (ed), The discovery of natural products with therapeutic potential. Butterworth-Heiniemann, Boston, pp.49-80

Ejike CM, Ezeanu CS and Eboatu AN(2014). Determination of physical and phytochemical constituents of some tropical timbers indigenous to Niger delta area of Nigera. European Scientific Journal 10(18):247-270

European Committee for Antibacterial Susceptibility Testing (EUCAST) of the European Socienty of Clinical Microbiology and Infectious Diseases (ESCMID) (2003). Determination of minimum inhibitory concentration (MIC) of antibacterial agents by broth dilution. Clinical Microbiology and Infection 9(8):1-7

Gimenez C, Cabrera R, Reina M and Gonzalez-Coloma A (2007). Fungal endophytes and their role in plant protection. Current Organic Chemistry 11: 707-720

Gunatilaka AAL (2006). Natural products from plant-associated microorganisms; distribution, structural diversity, bioactivity and implications of their occurrence. J Nat Prod 69: 509-526

Hardoim PR, Van Overbeek LS, Elsas JD (2008). Properties of bacterial endophytes and their proposed role in plant growth. Trends in microbiology 16(10): 463-471

Hoffman AM, Mayer SG, Strobel GA, Hess WM, Sovocool GW, Grange AH, Harper JK, Arif AM, Grant, DM and Kelley-Swift EG (2008). Purification, identification and activity of phomodione, a furandione from an endophytic Phoma species. Phytochemistry 69(4):1049-1056

Irinyi L, Gade AK, Ingle AP, Kovics GJ, Rai MK and Sandor E (2009). Morphology and molecular biology of Phoma. In Gherbawy Y, March RL, and Rai M (eds.) Current advances in Molecular Mycology. Nova Science Publishers inc., New York. Pp 171-203.

Jahangirian H, Haron MJ, Shay MH, Abdollai Y, Rezayi M and Vafaei N, (2013). Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthezised from canola and palm kernel oils. Digest Journal of Nanomaterials & Biostructures (DJNB) 8(3):1263-1270

Joseph B, and Priya RM (2011). Bioactive compounds from endophytes and their potentioal in pharmaceutical effect: a review. Am J Biochem Biotechnol 1: 291-309

Kjer J, Debbab A, Aly AH and Proksch P (2010). Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nature protocols 5(3): 479-490

Ong JE and Gong WK (2013). Structure, function and management of mangrove ecosystems. ISME Mangrove educational book series. No 2. International Society for Mangrove Ecosystem (ISME), Okinawa, Japan, and Internatioal Tropical Timber Organization (ITTO), Yokohama, Japan, pp. 7-24.

Petrini O (1991). Fungal endophytes of tree leaves. In: Andrews JH and Hirana SS (eds). Microbial ecology of leaves, Springer-Verlag, New York, pp179-197

Pravabathy D and Nachiyar CV (2011). Screening and Characterization of antimicrobial compound from endophytic Aspergillus sp. isolated from Ficus carica. Journal of Pharmacy Research 4(6): 34-38

Ramakrishnan G, Kothai R, Jaykar B and Rathnakumar TV (2011). In vitro Antibacterial Activity of different extracts of Leaves of Coldenia procumbens. International Journal of Pharm Tech Research 3:1000-1004

Ravindran C, Naveenan T, Varatharajan GR, Rajasabapathy R and Meena RM (2012). Antioxidants in mangrove plants and endophytic fungal associations. Botanica Marina 55: 269-279

Rodriguez RJ, Redman RS and Henson JM (2004). The role of fungal symbioses in the adaptations of plants to high stress environments. Mitigation and Adaptation Strategies for Global Change 9:261-272

Schulz B, Boyle C, Draeger S, Römmert AK and Krohn K (2002). Endophytic fungi: a source of novel biologically active secondary metabolites. Mycological research 106: 996–1004

Sivakumar T (2013). A review on biodiversity of marine and mangrove fungi. International Journal of Current Research and Academic Review 1(1):26-44

Strobel G, Daisy B and Dastillo U (2005). Novel natural products from rainforest endopnytes. In: Zhang L and Demain AL (eds) Natural product drug discovery and therapeutic medicine. Humana Press, Totowa, New Jersey, pp 329-351

Thatoi H, Behera BC and Mishra RR (2013). Ecological role and biotechnological potential of mangrove fungi: a review. Mycology: An International Journal of Fungal Biology 4(1):54-71

Trease GE and Evans WC (2002). Pharmacognosy. 15th Ed. London, Saunders Publishers, pp. 42–44, 221-229

Wikee S, Udayanga D, Crous PW, Chukeatirote E, McKenzie EHC, Bahkali AH, Dai D and Hyde KD (2011).Phyllosticta – a current view of species recognition. Fungal Diversity, 51(1):43-61