Preliminary Phytochemical and Cytotoxic Activity of The Aqueous and Chloroform Fractions of The Leaf of Persea Americana Mill (Lauraceae)
Barre latérale de l'article
Contenu principal de l'article
Résumé
The preliminary phytochemical and cytotoxic effects of the chloroform and aqueous fractions of the methanol extract of the leaves of Persea americana were carried out. Cytotoxicity was carried out using tadpoles of Ranniceps raninus at 20-400 ?g/ml. The methanol extract was subjected to solvent partitioning and chromatographic procedures guided by cytotoxic tests. Phytochemical screening results revealed the presence of saponins, flavonoids, tannins and cardiac glycosides .The chloroform fraction of the leaf extract produced 100 % tadpole mortality at 200 ?g/ml. Chromatographic analysis of the active chloroform fraction yielded a compound (CH-B2) which gave a tadpole mortality of 96.70 ±3.33 % at 10 ?g/ml. Cytotoxicity results showed that the chloroform fraction had an LC50 of 10.77 ?g/ml while the its VLC sub-fractions 4 and 5 and isolated compound had LC50 of 4.67, 18.0 and 3.33 ?g/ml respectively. The results support the folkloric use of the plant in treating tumor related ailments. However, further work need to be carried out to establish the identity of the isolated compound.
Téléchargements
Renseignements sur l'article
Cette œuvre est sous licence Creative Commons Attribution - Pas d'Utilisation Commerciale - Pas de Modification 4.0 International.
Références
Ayinde, B. A., Omogbai E.K.I and Ikpefan E.O (2011). Comparative cytotoxic and antiproliferative effects of Persea americana Mill, leaf, stem and root barks. Journal of Pharmaceutical Science. 10:16-26
Dai, J. and Mumper R.J (2010) . Plant Phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15: 7313-7352.
Evans WC (2002). Trease and Evans Pharmacognosy,15th edition. W.B Sauders Company Ltd, London. pp 137-139, 230-240.
Goldman, I., Kopelberg, M., Devaene, J., Schwartz, B (1996). Antiplatelet activity in onion is sulfur dependent. Thrombosis and Haemostasis. 450-452
Gordaliza MA (2007). Natural products as leads to anticancer drugs. Clinical and Translational Oncology 9, 767–776.
Khandelwal KR (2006). Practical Pharmacognosy. Pune: NiraliPrakashan, 149-56p.
Koduru S, Grierson DS, Afolayan AJ (2006). Antimicrobial activity of Solanum aculeastrum (Solanaceae). Pharmaceutical Biology. 44, 284-286
Leland JC, Ara K, Peter BKSL, James AD and Harry LB (2006). Natural products from plants, 2nd ed., Taylor & Francis Group. LLC.
Ma W.W., Anderson J.E., Chang C.J., Smith D.L., McLaughlin J.L (1989). Majorenolide and majorynolide: a new pair of cytotoxic and pesticidal alkene-alkyne delta-lactones from Persea major. Journal of Natural Products. 52(6):1263-1266.
Meurer-Grimes B, Mcbeth DL, Hallihan B, Delph S (1996). Antimicrobial activity in medicinal plants of the Scrophulariaceae and Acanthaceae. Pharmaceutical Biology 34, 243–248
Obuotor EM and Onajobi FD (2000). Preliminary evaluation of cytotoxic properties of Raphia hookeri fruit mesocarp. 1. Fitoterapia 71(2), 190-192.
Rabe T and Van Staden J (1997). Antibacterial activity of South African plants used for medicinal purposes. Journal of Ethnopharmacology 56, 81–87
Sarker M, Neckermann C and Muller O (2005). Assessing the health status of young AIDS and other orphans in Kampala, Uganda. Tropical Medicine & International Health 10 (Suppl. 3), 210–215
Sofowora A. (1993). Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd; Screening Plants for Bioactive Agents 134–156p