GC-MS chemical profiling and anti-inflammatory effects of dichloromethane fractions from Dracaena arborea (Willd.) Link Asparagaceae in mice
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Abstract
Background: This study aimed to investigate the phytochemical composition and anti-inflammatory properties of dichloromethane fractions derived from the leaf, stem, and root of Dracaena arborea.
Methods: Plant materials were collected, dried at 40ºC, and powdered. The powders were macerated with 70% ethanol for 72 hours, and the extracts were concentrated under reduced pressure. After defatting with n-hexane, the extracts were partitioned with dichloromethane, yielding fractions for leaf (Dal), stem (Das), and root (Dar). Phytochemical analysis was conducted using Gas Chromatography-Mass Spectrometry (GC-MS), and the anti-inflammatory effects were evaluated using egg albumin- and xylene-induced oedema models in mice.
Results: The fractions significantly reduced topical oedema (p?0.05) in mice compared to dexamethasone (4 mg/kg), a standard anti-inflammatory drug. GC-MS analysis identified bioactive compounds with known anti-inflammatory properties, including 9,12-octadecadienoic acid, 9,12,15-octadecatrienoic acid, n-hexadecanoic acid ethyl ester, oleic acid, and gamma-sitosterol.
Conclusion: The presence of these lipophilic compounds in the leaf, stem, and root of D. arborea may account for the observed anti-inflammatory effects, highlighting the plant’s potential therapeutic value.
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References
Illodibia, C, Ugwu R, Okeke C, Akachukwu, E., Aziagba, B., Okeke, N. (2015). Anatomical studies on two species of Dracaena in South Eastern Nigeria. International Journal of Biological Research; 3(1): 9-15.
Ogunmodede OS, Oseni SO, Adenmosun OO and Oyekan, JO. Dracaena arborea Leaf Extract: a Phytotherapeutic Option for Ameliorating Oxidative Stress-mediated Endocrine and Testicular Disorders in Alloxan-induced Diabetic Rats. J Coast Life Med. 2015; 3(10): 809 – 814
Watcho P, Modeste WN, Albert K and Carro-Juarex M. Dracaena arborea Extracts Delay the Pro-Ejaculatory effect of Dopamine and Oxytocin in Spinal Male Rats. Int J Impot Res. 2014; 6: 213 – 217
Burkhill HM. The Useful Plants of West Tropical Africa, Volume 3. Kew, United Kingdom: Royal Botanic Garden. 1985; 880 p.
Umoh UF, Andy NA, Igwe MA, Ubengama EE, Obasi OI and Usen IE. Comparative phytochemical profiling and antioxidant activity of ethanol extracts of three parts of Dracaena arborea (Willd.) Link. Dutse Journal of Pure and Applied Sciences. 2024;10 (3c): 55-63
Echavarria AP, D’Avmas H, Matute N and Cano JA. Phytochemical analyses of eight plants from two provinces of Ecuador by GC-MS. Int J Herb Med. 2020; 8: 10-20.
Truong V, Bae Y, Rarison RHG, Bang J, Park S and Jeong W. Anti-Inflammatory and antioxidant activities of lipophilic fraction from Liriope platyphylla seeds using network pharmacology, molecular docking, and in vitro experiments. Int J Mol Sci. 2023; 24(19): 14958
Kediso TE, Tolessa T, Getachew F, Makonnen E. and Seifu D (2021). Effect of 70% ethanol extract and its solvent fractions of Artemisia afra (Jacq. Ex Willd.) against pentylenetetrazole-induced seizure in mice. Evid Based Complement Alternat Med. 2021; 2021: 6690965.
Umoh UF, Thomas PS, Okokon JE, Ajibesin KK and Eseyin OA. Comparative study on anti-inflammatory and analgesic effects of the leaf stem, and root of Dracaena arborea (Wild) Linn. (Asparagaceae). Nig J Pharm App Sc Res. 2020; 9(2): 40-46.
Clark JD, Gebhart GF, Gonder JC, Keeling ME and Kohn DF. The 1996 guide for the care and use of laboratory animals. ILAR Journal. 1997; 38 (1): 41-48
Dosso K, Attemene DSD, Gboko AO, N’guessan BB and Yapo AP. Evaluation of anti-inflammatory and healing activities of dichloromethane fraction of ethanol extract of stem bark of Piliostigma recticulatum. African Journal of Biological Sciences. 2020; 2 (3); 72-80.
Singsai K, Charoongchit P, Chaikaew W, Boonma N, Phanjaksai P, Chaisatan K. Antilipoxygenase and anti-inflammatory activities of Streblus asper leaf extract on xylene-induced ear edema in mice. Advances in Pharmacological and Pharmaceutical Sciences. 2020; 3176391: 1-5.
Muhammad N, Khan R, Seraj F, Khan A, Ullah U, Wadood A, Ajmal A, Ali B, Khan KM. Nawaz NUA, Almasoud N, Alomar TS and Rauf A. Invivo analgesic, anti-inflammatory and molecular docking studies of s-naproxen derivatives. Trends in Immunology (Neuroimmunology 11). 2024; n10 (2): e24267.
Morais SM, Nascimento JET, Sousasilva AA, Junior JER, Pinheiro DCS and Oliveira RV. Fatty acid profile and anti-inflammatory activity of fixed plant oils. Acta Scienntiae Veterinariae. 2017; 45 (1437): 1-8.
Dandekar R, Fegade B and Blaskar VH. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. J Pharm Phytochem. 2015; 4 (1): 149-154.
Aparna V, Vijayan D, Mandol P and Haridas M. Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chemical Biology and Drug Design. 2012; 80 (3): 434-439.
Sermakkani M and Thangapandian V. GC – MS analysis of Cassia italic leaf methanol extract. Asian J Pharm Cl Res. 2012; 5(2): 90 – 94.
Watcho P, Tchatat BYP, Deeh PBD, Fozin GRB and Wankeu-Nya M. Dracaena arborea improves sperm characteristics and antioxidant enzymes in adult male rats with experimental varicocele. JBRA Assist Reprod. 2021; 25 (3): 329-336.
Zeinab A. Kandil, ZA, Esmat, A, El-Din, RS and Ezzat, SM. Anti-inflammatory activity of the lipophilic metabolites from Scolymus hispanicus L. South African Journal of Botany. 2020; 131: 43-50.