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Background of Study: Genotoxicity describes the characteristic of chemical substances known as
genotoxins that harm the genetic material inside a cell, resulting in mutations that may predispose to
Methods: This research utilized a cross sectional study design aimed at determining possible association
between lifestyle factors and genotoxicity of undergraduate subjects in Benin City. One hundred and
ninety five (195) subjects, involving one hundred and fifty (150) undergraduates as study subjects and
forty-five (45) intending students as control participated. Questionnaires were used for data collection
while a wooden spatula was used to obtain buccal epithelial cells for micronuclei investigation. The
presence of micronuclei (MN) was assessed under light microscopy and a total of 200 cells per sample
Results: The results showed that there was no significant statistical difference (p>0.05) between the mean
micronuclei (Mni) cells counts of the non-cigarette smokers and former smokers; However, the average
Mni count for Shisha smokers was statistically significantly lower than non-shisha smokers. The average
Mni count between alcohol consumers and non-consumers was not statistically significant. The mean
micronuclei (Mni) count per 200 cells was (5.56±1.30) in undergraduate compared to intending students
(3.72±1.39), the mean Mni in age group ? 20yrs was 4.32±1.39 while the average Mni for ? 20yrs of age
was (2.82±1.29) with each of these groups being statistically significant (p-values =0.02).
Conclusion: There is a clear correlation between lifestyle factors and genotoxicity in undergraduate
subjects in this study as observed from the increase in buccal cell micronucleus.
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