The Antidiarrheal Activities of Methanol extract of <em>Holarrhena Floribunda</em> on rodents

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A. E. Udoh
P.A. Nwafor
A.S. Udobre

Abstract

The antidiarrheal activities of methanol extract of H.floribunda on rats were investigated using castor oil-induced diarrhea, small intestinal transit time and castor oil-induced fluid accumulation models. In the castor oil induced diarrhea theresults showed that the extract(110.22mg/kg)alone produced a mean fecal matter of 10.83±0.82 translating to 69.05% inhibition of the diarrhea .The percentage inhibition was significant[P?0.01]. When Yohimbine was combined with the extract, the mean fecal matter increased from 10.83±0.82 to 25.16±1.6 (28.11%) compared to control. This shows that Yohimbie, an ?2- adrenoceptor antagonist, antagonized the effect of the extract. The intestinal transit time significantly[P?0.01]decreased from 81.71±1.63 to 65.71±2.90 tranlating to 25.58% inhibition. As the dose of the extract increased from 36.74 to 110.22 mg/kg, the intestinal fluid accumulation decreased significantly[P?0.01] from 3.26±0.14 to1.55±0.10 translating to 13.75 to 58.99% reduction. Similarly, when Yohimbine was co-administered with the extract the intestinal fluid accumulation increased from1.55±0.10 (58.99%) to 1.88±0.13 (50.26%) fluid accumulation. The fact that Yohimine has antagonized the antidiarrheal effect of the extract suggests that the?2- adrenoceptor has a role to play in this antagonism. The percentage inhibition by Diphenoxylate, a standard antidiarrhea and a muscarinic blocker(67.14%) was higher than that of the combined doses of the extract (28.11%).Phytochemical analysis also shows that H.floribunda contain Phlobatanins, Flavonoids and alkaloids which might have also contributed to the antidiarrheal activities.

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Udoh, A. E., Nwafor, P., & Udobre, A. (2020). The Antidiarrheal Activities of Methanol extract of <em>Holarrhena Floribunda</em> on rodents. Nigerian Journal of Pharmaceutical and Applied Science Research, 1(2), 67–73. Retrieved from https://nijophasr.net/index.php/nijophasr/article/view/22
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References

Bogne, K. P., Penlap,B.V.,Lontsi, D (2007). Antibacterial Activities of the Extractsfrom Holarrhena Floribubda: African Journal of

Traditional Complementary and Alternative Medicine 4 (3):352-356.

Burkill, H.M 1997. The Useful Plants of West Tropical Africa. (4):59-103 Chukwura, B. k. C., 1997. Antimicrobial Activity of Holarhena Floribunda Stem Bark Ethanol Extract. Fitotherapia 68 (2):180-181. Dept of Pharm. Chem. University of Nigeria, Nsukka, Nigeria.

Costello, A. M., Bhutta, T.I. . 1992: AntidiarrhealDrugs for Acute Diarrhea in children. British Medical Journal(304) 1-2. Donald, G.S., 1994. Drugs used in Gastro Intestinal disorders. Modern pharmacology. (4)823 -838.

Farthing, M., Feldman, R., Finch, R. (1996). The Management of Infective gastroenteritis in adults.Journal of Infections.33(3)142-152.

Hutchinson, J. andDalziel, J.M.,(1972). Flora of West Tropical Africa. (2)300 -400. Crown Agents, London.

Jousilahti, P., Madkour, S.M., Lambrechts,T andSnerwin,E. (1997). Diarrheal disease morbidity and home treatment practical. Egypt Public Health,111(1):5-10.

Kagbo, H.D. and Eyaru, S.G.(2012). Evaluation of Antidiarrheal Potential of Methanol Extract of Garcinia kola stem bark. International Journal of Pharmaceutical Research and Development, pp136-142.

Kumar, P. and Clark, M. (2006). Clinical Medicine (6):200-300. S.W. Saunders. Philadelphia.

Lorke P. D., 1983. A New Approach to Practical Acute Toxicity Testing. ArchivesToxicity(53): 275-289.

Nwafor, P.A.,Okwuasaba, F.K. and Onoruvwe, O.O (2005). Effect of Methanol Extract of Asparagus Pubescens Root on non-Vascular Smooth Muscle in Experimental Animals. West African Journal of Pharmacology and Drug Research (21):31 -36.

Nwodo, O.F., Alumanah, E.O.(1991). Studies on Aburs Precarious Seed Antidarrheal Activity.Journal of Ethno Pharmacology 311: 395-398.

Rang, H. P.,Dale, M.M.Ritter, J.M. Moore, P.K. (2003). Pharmacology. (5): 367- 379

Robert, A.,Nezamis, J.F.Lancaster, CHanchar, A.J. Lepper, M.S. (1996). Enterpooling Assay; a Test for Diarrhea Produced by Prostaglandins. Prostaglandins. 11: 809- 8828

Sofowora, A. (1993).Medicinalplant and Traditional Medicine in Africa. (2):159 -177 Spectrum Book Ltd. Ibadan, Nigeria.

Trease, A. and Evans, W(2009).Trease and Evans Pharmacognosy(15):256-519 Bailhere, Tindall London.

Walker, R. and Whittlesea, C. (2002). Diarrhea. Clinical Pharmacy and Therapeutics, UK: Livingstone Churchill, 5:214-221