Evaluation of the antidiarrhoeal and ex-vivo intestinal activity of Hymenocardia acida Tul. (Phyllanthaceae) methanol leaf extract and chloroform fraction using animal models
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Abstract
Hymenocardia acida leaves and stem-bark are used traditionally for the management of diarrhoea and dysentery. This study was therefore aimed at investigation of the leaf extract and fraction on diarrhoea and intestinal motility. The leaves were collected, powdered, macerated in methanol for 72 h, and concentrated under pressure. Liquid-liquid partitioning was additionally performed yielding chloroform and aqueous fractions. The crude methanol extract (MHA) and the chloroform fraction (CHA) were then analysed. Qualitative phytochemical analyses on MHA were performed using standard procedures. The activity of MHA (100, 200 and 400 mg/kg) on intestinal transit, castor oil-induced diarrhoea and castor-oil induced enteropooling was assessed with atropine or loperamide (5 mg/kg) as positive control. MHA and CHA were further examined on the isolated rat ileum motility. Several secondary metabolite classes were detected. MHA had no significant effect (P > 0.05) on intestinal transit while producing significant inhibition of castor-oil induced diarrhoea (P < 0.001). MHA had no significant effect (P > 0.05) on intestinal fluid accumulation. MHA inhibited the amplitude and frequency of spontaneous intestinal contractions; however, CHA increased the amplitude but inhibited the frequency. Both MHA and CHA inhibited acetylcholine-induced intestinal contractions though MHA showed significant inhibition (P< 0.001) of the frequency. This study has shown that H. acida leaves have anti-diarrhoea and anti-motility activities supporting its use traditionally.
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References
Abu, A.H., Uchendu, C.N., 2011. Effect of aqueous ethanolic extract of Hymenocardia acida stem bark on oestrous cycle of albino rats. J. Med. Plants Res. 5, 1280–1283. doi:10.5897/JMPR10.407
Adeyemi, O.O., Akindele, A.J., 2008. Antidiarrhoeal activity of the ethyl acetate extract of Baphia nitida (Papilionaceae). J. Ethnopharmacol. 116, 407–412. doi:10.1016/j.jep.2007.12.004
Adeyemi, O.O., Akindele, A.J., Ogunleye, E.A., 2009. Evaluation of the antidiarrhoeal effect of Sanseviera liberica Gerome & Labroy (Agavaceae) root extract. J. Ethnopharmacol. 123, 459–463. doi:10.1016/j.jep.2009.03.023
Akindele, A.J., Salako, O.A., Ohonbamu, U. V., 2014. Evaluation of the antidiarrhoeal activity of the hydroethanolic leaf extract of Pupalia lappacea Linn. Juss. (Amaranthaceae). J. Ethnopharmacol. 151, 984–989. doi:10.1016/j.jep.2013.12.013
Bais, S., Gill, N.S., Shandil, S., 2014. Antidiarrhoel activity of methanolic extract of Achillea millefolium L. leaves in albino rats. Der Pharm. Lett. 6, 308–314.
Baldi, F., Bianco, M.A., Nardone, G., Pilotto, A., Zamparo, E., 2009. Focus on acute diarrhoeal disease. World J. Gastroenterol. 15, 3341–3348. doi:10.3748/wjg.15.3341
Beubler, E., Juan, H., 1979. Effect of ricinoleic acid and other laxatives on net water flux and prostaglandin E release by the rat colon. J. Pharm. Pharmacol. 31, 681–685. doi:10.1111/j.2042-7158.1979.tb13628.x
Bova, S., Saponara, S., Rampa, A., Gobbi, S., Cima, L., Fusi, F., Sgaragli, G., Cavalli, M., De Rios, C.L., Striessnig, J., Bisi, A., 2009. Anthracene based compounds as new L-type Ca2+ channel blockers: Design, synthesis, and full biological profile. J. Med. Chem. 52, 1259–1262. doi:10.1021/jm801589x
Bretagne, J.F., Vidon, N., L’Hirondel Ch., Bernier, J.J., 1981. Increased cell loss in the human jejunum induced by laxatives (ricinoleic acid, dioctyl sodium sulphosuccinate, magnesium sulphate, bile salts). Gut 22, 264–269.
Burkill, H.M., 1985. The useful plants of west tropical Africa, 2nd ed. Royal Botanic Gardens, Kew, UK. doi:10.1097/NAQ.0b013e318258ba14
Capasso, F., Mascolo, N., Izzo, A.A., Gaginella, T.S., 1994. Dissociation of castor oil-induced diarrhoea and intestinal mucosal injury in rat: effect of NG-nitro-L-arginine methyl ester. Br. J. Pharmacol. 113, 1127–1130. doi:10.1111/j.1476-5381.1994.tb17113.x
Casburn-Jones, A.C., Farthing, M.J., 2004. Management of infectious diarrhoea. Gut 53, 296–305.
Chang, E.B., Leung, P.S., 2014. Gastrointestinal Motility, in: The Gastrointestinal System. Springer, Netherlands, pp. 35–62.
Ezeigbo, I.I., Asuzu, I.U., 2012. Anti-diabetic activities of the methanol leaf extracts of Hymenocardia acida (Tul.) in alloxan-induced diabetic rats. African J. Tradit. Complement. Altern. Med. 9, 204–209.
Gullikson, G.W., Cline, W.S., Lorenzsonn, V., Benz, L., Olsen, W.A., Bass, P., 1977. Effects of anionic surfactants on hamster small intestinal membrane structure and function: relationship to surface activity. Gastroenterology 73, 501–511.
Harborne, J.B., 1998. Phytochemical Methods; A Guide to Modern Techniques of Plant Analysis, Journal of Chemical Information and Modeling. doi:10.1017/CBO9781107415324.004
Heel, R.C., Brogden, R.N., Speight, T.M., Avery, G.S., 1978. Loperamide: A Review of its Pharmacological Properties and Therapeutic Efficacy in Diarrhoea. Drugs 15, 33–52. doi:10.2165/00003495-197815010-00003
Hsu, W.H., 1982. Xylazine-induced delay of small intestinal transit in mice. Eur. J. Pharmacol. 83, 55–60. doi:10.1016/0014-2999(82)90285-0
Irvine, F.R., 1961. Woody plants of Ghana, Ist. ed. Oxford University Press, London.
Kavitha, D., Shilpa, P.N., Niranjali Devaraj, S., 2004. Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica Wall. Indian J. Exp. Biol. 42, 589–594.
Mishra, A., Seth, A., Maurya, S., 2016. Therapeutic significance and pharmacological activities of antidiarrhoeal medicinal plants mention in Ayurveda: A review. J. Intercult. Ethnopharmacol. 5, 290. doi:10.5455/jice.20160426094553
Mishra, A., Sharma, V., Hem, K., Maurya, S.K., 2015. Plants used for treatment of diarrhea: An ayurvedic prospective. Innov. J. Ayurvedic Sci. 3, 1–6.
Nakao, S., 1963. The effect of ricinoleic acid and phenolphthalein on creatine phosphate levels in the rat intestine in vitro. Biochemistry 12, 216–217.
National Research Council, 2010. Guide for the Care and Use of Laboratory Animals: Eighth Edition, in: Guide for the Care and Use of Laboratory Animals. National Academies Press, p. 118. doi:10.2307/1525495
NIH, 2015. Public health service policy on humane care and use of laboratory animals, Office of Laboratory Animal Welfare.
Oben, J.E., Assi, S.E., Agbor, G.A., Musoro, D.F., 2006. Effect of Eremomastax speciosa on experimental diarrhoea. African J. Tradit. Complement. Altern. Med. 3, 95–100.
Odebiyi, O.O., Sofowora, E.A., 1978. Phytochemical screening of Nigerian medicinal plants II. J. Nat. Prod. 41, 234–246.
Page, A.-L., Hustache, S., Luquero, F.J., Djibo, A., Manzo, M.L., Grais, R.F., 2011. Health care seeking behavior for diarrhea in children under 5 in rural Niger: results of a cross-sectional survey. BMC Public Health 11, 389. doi:10.1186/1471-2458-11-389
Phillips, R.A., Love, A.H.G., Mitchell, T.G., Neptune, E.M., 1965. Cathartics and the sodium pump. Nature 206, 1367–1368.
Pinto, A., Autore, G., Mascolo, N., Sorrentino, R., Biondi, A., Izzo, A.A., Capasso, F., 1992. Time Course of PAF Formation by Gastrointestinal Tissue in Rats After Castor Oil Challenge. J. Pharm. Pharmacol. 44, 224–226. doi:10.1111/j.2042-7158.1992.tb03586.x
Saunders, D.R., Sillery, J., Suruwica, C., Tytgat, G.N., 1978. Effect of phenolphthalein on the function and structure of rodent and human intestine. Dig. Dis. Sci. 23, 909–913.
Schiller, L.R., 2012. Definitions, pathophysiology, and evaluation of chronic diarrhoea. Best Pract. Res. Clin. Gastroenterol. 26, 551–562. doi:10.1016/j.bpg.2012.11.011
Simon, B., Kather, H., 1980. Interaction of laxatives with enzymes of cyclic AMP metabolism from human colonic mucosa. Eur. J. Clin. Invest. 10, 231–234.
Skadhauge, E., Grondal, M.L., Hansen, M.B., 1997. Pathophysiology and symptomatic treatment of secretory and osmotic diarrhoea, in: Digestive Physiology in Pigs. pp. 241–253.
Sofidiya, M.O., Odukoya, O.A., Adedapo, A.A., Mbagwu, H.O.C., Afolayan, A.J., Familoni, O.B., 2010. Investigation of the anti-inflammatory and antinociceptive activities of Hymenocardia acida Tul. (Hymenocardiaceae). African J. Biotechnol. 9, 8454–8459.
Sofowora, A., 1993. Recent trends in research into African medicinal plants. J. Ethnopharmacol. 38, 197–208.
Taiwo, B.J., Igbeneghu, O.A., 2014. Antioxidant and antibacterial activities of flavonoid glycosides from Ficus exasperata Vahl-Holl (moraceae) leaves. African J. Tradit. Complement. Altern. Med. 11, 97–101.
Teke, G.N., Kuiate, J.-R., Kueté, V., Teponno, R.B., Tapondjou, L.A., Vilarem, G., 2010. Antidiarrheal activity of extracts and compound from Trilepisium madagascariense stem bark. Indian J. Pharmacol. 42, 157–63. doi:10.4103/0253-7613.66839
Trease, G.E., Evans, W.C., 1989. Phenols and phenolic glycosides, in: Textbook of Pharmacognosy. Balliese, Tindall and Co Publishers, London, pp. 343–383.
Uwumarongie, H., Enike, M.A., Bafor, E.E., 2016. Pharmacognostic Evaluation and gastointestinal activity of Dryopteris Filix-Mas (L.) Schott (Dryopteridaceae). Ewemen J. Herb. Chem. Pharmacol. Res. 2, 19–25.
Whyte, L.A., Jenkins, H.R., 2012. Pathophysiology of diarrhoea. Paediatr. Child Health (Oxford). doi:10.1016/j.paed.2012.05.006