PROINFLAMMATORY EFFECTS OF LAUNAEA TARAXACIFOLIA AQUEOUS LEAVES EXTRACT IN BENZENE INDUCED HAEMATOTOXICITY IN ALBINO MALE WISTAR RATS

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Progress Arhenrhen Obazelu
Favour E. Wasa

Abstract

Background: Haematotoxicity induced by benzene exposure has been associated with altered gene expressions of proinflammatory cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-?). Launaea taraxacifolia, a plant known for its traditional medicinal uses, has emerged as a subject of interest due to its reported anti-inflammatory properties. Therefore, the aim of this study was to determine the proinflammatory effects of aqueous leaf extracts of Launaea taraxacifolia on benzene-induced haematotoxicity in albino Wistar rats.


Methods: A total of sixty (60) adult male albino Wistar rats were divided into six (6) groups, namely groups A, B, C, D, E, and F, representing control, benzene group, cyclophosphamide group, benzene + 100mg/kg Launaea taraxacifolia, benzene + 200mg/kg  Launaea taraxacifolia, and benzene + 400mg/kg  Launaea taraxacifolia, respectively. mRNA expressions of IL-6 and TNF-? were determined using polymerase chain reaction (PCR). The data obtained was analysed by the Statistical Package for Social Science (SPSS) software.


Results: mRNA expression of IL-6 in group B was significantly higher when compared to group A (p<0.05).The mRNA expression of IL-6 was significantly higher in groups E and F when compared to group A (p<0.05). There was a significant increase in the mRNA expression of TNF-? in group B when compared to group A (p<0.05).


Conclusion: This study concludes that benzene exposure induces an increase in the mRNA expression of IL-6 and TNF-?. Co-administration of benzene and varying Launaea taraxacifolia concentrations subsequently reduced TNF-?, while a further increase was noted in IL-6 expression after administration of 200mg/kg with benzene.

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How to Cite
Obazelu, P. A., & E. Wasa, F. (2024). PROINFLAMMATORY EFFECTS OF LAUNAEA TARAXACIFOLIA AQUEOUS LEAVES EXTRACT IN BENZENE INDUCED HAEMATOTOXICITY IN ALBINO MALE WISTAR RATS. Nigerian Journal of Pharmaceutical and Applied Science Research, 12(4), 34–40. Retrieved from https://nijophasr.net/index.php/nijophasr/article/view/528
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References

Gurib-Fakim, A. (2006). Medicinal Plants: Traditions of Yesterday and Drugs of Tomorrow. Molecular Aspects of Medicine, 27(1): 1-93.

Saxena, M., Saxena, J., Nema, R., Singh, D., Gupta, A. (2013). Phytochemistry of Medicinal Plants. Journal of Pharmacognosy and Phytochemistry, 1(6): 168-182.

Bernhoft, A. J. A. B. (2010). A Brief Review on Bioactive Compounds in Plants. Bioactive Compounds, 50(1): 11-17.

Dansi, A., Vodouhè, R., Azokpota, P., Yedomonhan, H., Assogba, P., Adjatin, A., Akpagana, K. J. T. S. W. J. (2012). Diversity of the Neglected and Underutilized Crop Species of Importance in Benin. The Scientific World Journal, 5(2): 23-28.

Adjei, D. G. G., Mireku-Gyimah, N. A., Sarkodie, J. A., Nguessan, B. B., Kodua, E., Amedior, J. K., Nyarko, A. K. (2022). Antidiabetic Properties of an Ethanolic Leaf Extract of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey (Asteraceae) in Rats. Clinical Phytoscience, 8(1): 1-15.

Kumar, S., Bajwa, B. S., Kuldeep, S., Kalia, A. N. (2013). Anti- Inflammatory Activity of Herbal Plants: A Review. International Journal of Advances in Pharmacy, Biology and Chemistry, 2(2): 272-281.

Omoigui, S. (2007). The Biochemical Origin of Pain–Proposing a New Law of Pain: The Origin of all Pain is Inflammation and the Inflammatory Response. Part 1 of 3–A Unifying Law of Pain. Medical Hypotheses, 69(1): 70-82.

Thompson, A. W., Lotze, M. T. (2003). The Cytokine Handbook, Volume 1, Chapter 1, Academic Press Publishing Company, Cambridge. Pp. 1-3.

Chang, J. H., Ryang, Y. S., Morio, T., Lee, S. K., Chang, E. J. (2004). Trichomonas vaginalis Inhibits Pro-Inflammatory Cytokine Production in Macrophages by Suppressing NF-Kappa B Activation. Molecules and Cell, 18: 177–185.

Bari, M. A., Kindzierski, W. B., Wheeler, A. J., Héroux, M. È., Wallace, L. A. (2015). Source Apportionment of Indoor and Outdoor Volatile Organic Compounds at Homes in Edmonton, Canada. Building and Environment, 90(1): 114-124.

Snyder, R. (2012). Leukemia and Benzene. International Journal of Environmental Research and Public Health, 9(8): 2875-2893.

Lenz, A., Franklin, G. A., Cheadle, W. G. (2007). Systemic Inflammation after Trauma. Injury, 38(12): 1336-1345.

Minciullo, P. L., Navarra, M., Calapai, G., Gangemi, S. (2014). Cytokine Network Involvement in Subjects Exposed to Benzene. Journal of Immunology Research, 2(1): 3-8.

Obazelu, P. A., Faluyi, O. (2023). Effect of Launaea taraxacifolia Aqueous Leaves Extracts on ICAM-1 and VCAM-1 Gene Expressions in Benzene-Induced Haematotoxicity in Albino Wistar Rats. Journal of Applied Science and Environmental Management, 27(12): 2845-2852.

Obazelu, P. A., Aruomaren, A. I., Ugboaja, E.E. (2021). ‘Phytochemical Analysis, Nutrients and Mineral Composition of Combretum platypterum Aqueous Leaf Extract’. Journal of Applied Science and Environmental Management, 25(9): 1625-1630.

Akanni, E. O., Adedeji, A. L., Adedosu, O. T., Olaniran, O. I., Oloke, J. K. (2014). Chemopreventive and Anti-Leukemic Effects of Ethanol Extracts of Moringa Oleifera Leaves on Wistar Rats Bearing Benzene Induced Leukemia. Current Pharmaceutical Biotechnology, 15(6): 563-568.

Elekofehinti, O. O., Lawal, A. O., Ejelonu, O. C., Molehin, O. R., Famusiwa, C. D. (2020). Involvement of Fat Mass and Obesity Gene (FTO) in the Anti-Obesity Action of Annona muricata annonaceae: in Silico and in Vivo Studies. Journal of Diabetes and Metabolic Disorders, 19(1): 197-204.

Hooper, L., Cassidy, A. (2006). A Review of the Health Care Potential of Bioactive. Journal of the Science of Food and Agriculture, 86(12): 1805-1813.

Bahadar, H., Mostafalou, S., Abdollahi, M. (2014). Current Understandings and Perspectives on Non-Cancer Health Effects of Benzene: A Global Concern. Toxicology and Applied Pharmacology, 276(2): 83-94.

Ogbodo, J. O., Arazu, A. V., Iguh, T. C., Onwodi, N. J., Ezike, T. C. (2022). Volatile Organic Compounds: A Pro-Inflammatory in Autoimmune Diseases. Frontiers in Immunology, 13(1): 928379.

Samadi, M. T., Shakerkhatibi, M., Poorolajal, J., Rahmani, A., Rafieemehr, H., Hesam, M. (2019). Association of Long-Term Exposure to Outdoor Volatile Organic Compounds (BTXS) with Pro-Inflammatory Biomarkers and Haematologic Parameters in Urban Adults: a Cross-Sectional Study in Tabriz, Iran. Ecotoxicology and Environmental Safety, 180(1): 152-159.

Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Zhao, L. (2018). Inflammatory Responses and Inflammation-Associated Diseases in Organs. Oncotarget, 9(6): 7204.

Tamie, N., Rui-Sheng, W., Elovaara, E., Park, S. S., Gelboin, H. V., Vainio, H. (1993). Cytochrome P450-Related Differences Between Rats and Mice in the Metabolism of Benzene, Toluene and Trichloroethylene in Liver Microsomes. Biochemical Pharmacology, 45(5): 1079-1085.

Klopcic, I., Dolenc, M. S. (2018). Chemicals and Drugs Forming Reactive Quinone and Quinone Imine Metabolites. Chemical Research in Toxicology, 32(1), 1-34.

Youn, G. S., Lee, K. W., Choi, S. Y., Park, J. (2016). Over Expression of HDAC6 Induces Pro-Inflammatory Responses by Regulating ROS-MAPK-NF-?B/AP-1 Signaling Pathways in Macrophages. Free Radical Biology and Medicine, 97(1): 14-23.

Adinortey, M. B., Sarfo, J. K., Kwarteng, J., Adinortey, C. A., Ekloh, W., Kuatsienu, L. E., Kwadwo, N. A. (2018). The Ethnopharmacological and Nutraceutical Relevance of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey. Evidence-Based Complementary and Alternative Medicine, 2(1): 5-10.