Hippadine from Crinum jagus is active against ovarian carcinoma and melanoma cancer cell lines.

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Comfort Alicha Ogah
Dr. Celina Ogah
Dr. Olusegun S. Ajala
Prof. John Igoli
Alexander I. Grey
Valerie A. Ferro



Crinum jagus (Amaryllidaceae), has been used in folk medicine for the treatment of various diseases including cancer. The plant is rich in alkaloids and other phytoconstituents. This study aimed to isolate, and structurally elucidate compounds from Crinum jagus bulb (an Amaryllidaceae plant) and study the cytotoxic activity of hippadine on ovarian carcinoma (A2780) and melanoma cancer (A375) cell lines.


In this study, bulbs of C. jagus were extracted with methanol and fractionated with n-hexane, ethyl acetate, and methanol, Column chromatography was used to isolate and purify compounds while the identities of the compounds were confirmed using HRESI and 2D NMR spectroscopy. The cytotoxic activity of hippadine was evaluated on ovarian carcinoma (A2780) and melanoma cancer (A375) cell lines using a resazurin-based test (alamar blue assay).


Hippadine,  ?-sitosterol, stigmasterol, and 4’-hydroxy-7-methoxyflavan were isolated from the ethylacetate fraction of the bulbs. The identity of these compounds was further confirmed by comparing research data with other literature reports. This is an initial report of the flavan in Crinum jagus. The cytotoxic studies on hippadine gave IC50 values of 4.23± 0.35µg/ml on A2780 cells and 4.32 ± 0.55 µg/ml on A375 cells.


This study has justified the use of Crinum jagus bulb in folkloric medicine for the treatment of cancer. The phytochemical constituents hereby reported and the cytotoxic effect of hippadine provides a basis for further investigation into other pharmacological uses of Crinum jagus bulb as alkaloids are known for their diverse pharmacological activities.


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How to Cite
Ogah, C. A., Ogah, C. O., Ajala, O. S., Igoli, J. I., Gray, A., & Ferro, V. A. (2024). Hippadine from Crinum jagus is active against ovarian carcinoma and melanoma cancer cell lines. Nigerian Journal of Pharmaceutical and Applied Science Research, 12(4), 28–33. https://doi.org/10.60787/nijophasr-v12-i4-519
Author Biographies

Dr. Celina Ogah, University of Lagos

Associate Professor, 

Department of Pharmaceutical Chemistry, University of Lagos

Dr. Olusegun S. Ajala, University of Lagos

Senior Lecturer

Department of Pharmaceutical Chemistry

University of Lagos


Prof. John Igoli, Joseph Sarwuan Tarka University


Department of Chemistry,

Joseph Sarwuan Tarka University, Makurdi. 

Alexander I. Grey, University of Strathclyde



Strathclyde Institute of Pharmacy and Biomedical Sciences,

University of Strathclyde

Valerie A. Ferro, University of Strathclyde

Senior Lecturer

Strathclyde Institute of Pharmacy and Biomedical Sciences,

University of Strathclyde


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