Formulation and evaluation of effect of mucilage of Corchorus olitorius leaves on antioxidant activity of Spondias mombin extract
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Abstract
Background: Mucilage from medicinal plants such as Corchorus olitorius L. (Malvaceae) can serve as alternative sources of suspending agents as currently commercially available ones are quite costly. However, they may also affect formulation characteristics and activity of final product. This study evaluated effect of mucilage of Corchorus olitorius L. (Malvaceae) leaves on antioxidant activity of aqueous fraction of hydroethanolic extract of Spondias mombin L. (Anacardiaceae) leaves.
Methods: Standard in vitro antioxidant assays were used. Mucilage was investigated for its phytochemical contents. Physico-chemical evaluation using pH meter and microscopical characterization emulsion were also carried out. Repeated measures analysis of variance (ANOVA) was used to compare activity of extract versus emulsion.
Results: Terpenoids, unsaturated lactones, carbohydrates and reducing sugars were present in the mucilage. The stable emulsion formulated had a pH of 6.7 with oil droplets of average size, 9.12µm. Most droplets were small size. The extract had superior 2,2-diphenyl-1-picrylhydrazyl radical inhibition, hydrogen peroxide radical inhibition and reducing capacity (? = 0.05) but emulsion had better metal chelating activity (? = 0.05).
Conclusion: The mucilage significantly reduced antioxidant activity in the emulsion but could be used orally for indicated oxidative conditions.
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