Class 1 Integrons and Associated Gene Cassettes in Multidrug Resistant Acinetobacter baumannii from Southwest Nigeria: A Tertiary-Hospitals Study.

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Williams Ike
Bolanle A. Adeniyi
Joseph E. Peters



Background: Flooded soil is a significant agronomic problem because most plants do not tolerate excess water in the soil. Yenagoa, Bayelsa State is characterized by seasonal flooding that often engulf cultivated soils.  This study was conducted to assess the response of Phaseolus vulgaris to flooded soil condition in the study area.


Methods: Viable seeds of the test crop were sterilized and sown in soils of Site A (control) and B (flooded soil) in rows. The research work was planned such that, the P. vulgaris seedlings were established up to one (1) month before the area (Site B) was flooded. Plant growth parameters such as plant height, leaf number, dry weight, nitrogen, phosphorus and potassium contents were examined.   


 Results: P. vulgaris exhibited moderate wilting and chlorosis during the first week of exposure to flood condition. The severity of wilting and chlorosis increased with increase in the duration of the stress condition. Other morphological characteristics observed were retarded growth, dropping of leaves, decrease leaf number, and reduced leaf size during the second and third week of exposure to flood condition, and death of the entire plant at the fourth week of study. The leaf number and dry weight of the crop decreased with increase in the duration of exposure to flood condition with values comparatively lower than that of the control  


Conclusion: This study showed that P. vulgaris was susceptible to flooded soil. 





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Ike, W., A. Adeniyi, B. ., & E. Peters, J. . (2021). Class 1 Integrons and Associated Gene Cassettes in Multidrug Resistant Acinetobacter baumannii from Southwest Nigeria: A Tertiary-Hospitals Study. Nigerian Journal of Pharmaceutical and Applied Science Research, 10(1), 61-69. Retrieved from


Deylam SM, Ferdosi-Shahandashti E, Yahyapour Y, Khafri S, Pournajaf A, Rajabnia R. Integron-Mediated Antibiotic Resistance in Acinetobacter baumannii Isolated from Intensive Care Unit Patients, Babol, North of Iran. BioMed research international vol. 2017; 7157923.

Ayoub MC, Hammoudi HD. Insights into Acinetobacter baumannii: A Review of Microbiological, Virulence, and Resistance Traits in a Threatening Nosocomial Pathogen. Antibiotics 2020; 9(3):119.

Jaruratanasirikul S, Nitchot W, Wongpoowarak W, Samaeng M, Nawakitrangsan M. Population pharmacokinetics and Monte Carlo simulations of sulbactam to optimize dosage regimens in patients with ventilator-associated pneumonia caused by Acinetobacter baumannii. Eur. J. Pharm. Sci 2019; 136, 104940.

Bialvaei AZ, Kafil HS, Asgharzadeh M, Aghazadeh M, Yousefi M () CTX-M extended-spectrum ?-lactamase-producing Klebsiella spp, Salmonella spp, Shigella spp and Escherichia coli isolates in Iranian hospitals. Brazilian Journal of Microbiology 2016; 47(3):706–711.

Eftekhar F, Altayar F, Khidaei H. Plasmid-Mediated Class 1 and 2 Integron Carriage in Drug-Resistant Nosocomial Isolates of Acinetobacter baumannii. Arch Clin Infect Dis 2018; 13 (1):e57813.

Goudarzi M and Azimi H. Dissemination of Classes 1, 2, and 3 Integrons in Acinetobacter baumannii Strains Recovered from Intensive Care Units Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Jundishapur J Microbiol 2017; 10 (5): e13100.

Firoozeh F, Mahluji Z, Khorshidi A, and Zibaei M. Molecular characterization of class 1, 2 and 3 integrons in clinical multi-drug resistant Klebsiella pneumoniae isolates. Antimicrob Resist Infect Control 2019; 8:59.

Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME and Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J. Clin. Microbiol 2006; 44: 2974–2976.

CLSI, Performance Standards for Antimicrobial Susceptibility Testing: 29nd Informational Supplement CLSI M100-S29, CLSI, Wayne, PA, USA, 2019.

Ahangarkani F, Rajabnia R, Shahandashti E, Bagheri M, Ramez M. Frequency of class 1 integron in Escherichia coli strains isolated from patients with urinary tract infections in North of Iran. Mater Socio med 2015; 27(1):10–12.

Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ. Single-step selection of drug resistant Acinetobacter baylyi ADP1 mutants reveals a functional redundancy in the recruitment of multidrug efflux systems. PLoS One 2013; 8(2):e56090.

Khosravi Y, Tay ST and Vadivelu J. Analysis of integrons and associated gene cassettes of metallo-?-lactamase-positive Pseudomonas aeruginosa in Malaysia. J. Med. Microbiol 2011; 60: 988–994.

Chen DQ, Jiang YT, Feng DH, Wen SX, Su DH and Yang L. Integron mediated bacterial resistance and virulence on clinical pathogens. Microb Pathog 2018; 114: 453–457.

Xu L, Deng S, Wen W, Tang Y, Chen L, Li Y, Zhong G, Li J, Ting W, Fu B. "Molecular typing, and integron and associated gene cassette analyses in Acinetobacter baumannii strains isolated from clinical samples". Exp. Ther. Med 2020; 20 (3): 1943-1952.

Rafei R, Pailhoriès H, Hamze M, Eveillard M, Mallat H, Dabboussi F, Joly-Guillou ML, Kempf M. Molecular epidemiology of Acinetobacter baumannii in different hospitals in Tripoli, Lebanon using blaOXA-51-like sequence based typing. BMC Microbiol 2015; 15: 103.

Shi X, Wang H, Wang X, Jing H, Duan R, Qin S, Lv D, Fan Y, Huang Z, Stirling K, Zhang L and Wang J. Molecular characterization and antibiotic resistance of Acinetobacter baumannii in cerebrospinal fluid and blood. PLoS ONE 2021; 16 (2): e0247418.

Zhao S, Jiang D., Xu P, Zhang Y, Shi, H, Cao H, and Wu. Q. An investigation of drug-resistant Acinetobacter baumannii infections in a comprehensive hospital of East China. Ann Clin. Microbiol Antimicrob 2015; 14, 7.

Koczura R, Przyszlakowska B, Mokracka J, Kaznowski A. Class1 integrons and antibiotic resistance of clinical Acinetobacter calcoaceticus-baumannii complex in Poznan, Poland. Curr Microbiol 2014; 69(3):258–62.

Hu FP, Guo Y, Zhu DM, Wang F, Jiang XF, Xu YC, et al. Resistance trends among clinical isolates in China reported from CHINET surveillance of bacterial resistance, 2005–2014. Clinical microbiology and infection: the official publication of the European Society of Clinical Microbiology and Infectious Diseases 2016; 22 Suppl1: S9–14.

Hu FP, Guo Y, Yang Y, Zheng Y, Wu S, Jiang X., et al. Resistance reported from China antimicrobial surveillance network (CHINET) in 2018. European journal of clinical microbiology & infectious diseases: official publication of the European Society of Clinical Microbiology 2019; 38 (12): 2275–81.

Trebosc V, Gartenmann S, Totzl M, Lucchini V, Schellhorn B, Pieren M, et al. Dissecting Colistin Resistance Mechanisms in Extensively Drug-Resistant Acinetobacter baumannii Clinical Isolates. mBio 2019; 10 (4): e01083-19.

Bagheri JS, Moniri R, Firoozeh F, Sehat M, Dasteh GY. Susceptibility pattern and distribution of oxacillinases and blaPER-1 genes among multidrug resistant Acinetobacter baumannii in a teaching hospital in Iran. J Pathog 2015: 957259.

Tafreshi N, Babaeekhou L, and Ghane M. Antibiotic resistance pattern of Acinetobacter baumannii from burns patients: increase in prevalence of blaOXA-24-like and blaOXA-58-like genes. Iran J Microbiol 2019; 11(6):502-509.

Afhami S, Ali BM, Esmailpour BN, Saffar H, Hadadi A, Jafary NM et al. Antimicrobial resistance pattern of Acinetobacter; a multicenter study, comparing European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute (CLSI); evaluation of susceptibility testing methods for polymyxin. Immunopathol Persa 2021; 7 (1):e04.

Tewari R, Chopra D, Wazahat R, Dhingra S, and Dudeja M. Antimicrobial Susceptibility Patterns of an Emerging Multidrug Resistant Nosocomial Pathogen: Acinetobacter baumannii. Malays J Med Sci 2018; 25 (3): 129-134.

Jaggi N, Sissodia P, Sharma L. Acinetobacter baumannii isolates in a tertiary care hospital: antimicrobial resistance and clinical significance. JMID 2012; 2(2):57–63.

Van TD, Dinh, QD, Vu PD, Nguyen TV, Pham CV, Dao TT, et al. Antibiotic susceptibility and molecular epidemiology of Acinetobacter calcoaceticus–baumannii complex strains isolated from a referral hospital in northern Vietnam. J Glob Antimicrob Resist 2014; 2(4):318–321.

Boone RL., Whitehead B., Avery, T.M. et al. Analysis of virulence phenotypes and antibiotic resistance in clinical strains of Acinetobacter baumannii isolated in Nashville, Tennessee. BMC Microbiol 2021; 21, 21.

Bulens SN, Yi SH, Walters MS, Jacob JT, Bower C, Reno J, Wilson L, Vaeth E, Bamberg W, Janelle SJ, Lynfield R, Vagnone PS, Shaw K, Kainer M, Muleta D, Mounsey J, Dumyati G, Concannon C, Beldavs Z, Cassidy PM, Phipps EC, Kenslow N, Hancock EB, Kallen AJ. Carbapenem-Non-susceptible Acinetobacter baumannii, 8 US Metropolitan Areas, 2012-2015. Emerg Infect Dis 2018; 24(4):727–34.

Ike WE, Adeniyi BA, Peters JE. Molecular basis of Carbapenem-Resistant Acinetobacter baumannii from Southwest, Nigeria. NIJOPHASR 2020; 9 (2): 26–32.

Chen J, Li H, Yang J, Zhan R, Chen A, Yan Y. Prevalence and Characterization of Integrons in Multidrug Resistant Acinetobacter baumannii in Eastern China: A Multiple-Hospital Study. Int J Environ Res Public Health 2015; 12 (8): 10093-105.

Nemec A, Dolzani L, Brisse S, van den Broek P, Dijkshoorn L. Diversity of aminoglycoside-resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clones. J Med Microbiol 2004; 53 (Pt 12):1233-40.

Mirnejad R, Heidary M, Bahramian A, et al. Evaluation of Polymyxin B Susceptibility Profile and Detection of Drug Resistance Genes among Acinetobacter baumannii Clinical Isolates in Tehran, Iran during 2015–2016. Mediterr J Hematol Infect Dis 2018; 10 (1):e2018044.