Amelioration of Isoniazid induced Oxidative Stress and Hematotoxicity by Vitamin C in Wistar Rats

Main Article Content

Bilkisu B. Maiha
Dr Aliyu Bako
Sherifat Anafi
Muhammad S. Yusuf
Abdulkadir M. Kabiru


Background: This study was carried out to investigate the ameliorative effect of vitamin C against isoniazid-induced toxicity in some hematological and biochemical parameters in Wistar rats.


Methods: A total of seventy-two (72) adult Wistar rats of both sexes were used which were randomly distributed into 6 groups of 12 rats each. Isoniazid (INH) and Vitamin C were orally administered daily for 2 weeks and on the 7th and 14th day of the experiment, 6 rats from each group were humanely euthanized using ethyl ether and blood was collected for determination of antioxidant, and hematological parameters.


Results: This study revealed significant increase in the level of Malondialdehyde (MDA) in rats treated with INH only. These markers were significantly reduced following co-administration with vitamin C (10 mg/kg and 20 mg/kg) respectively. There was also significant reduction in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH) in rats treated with INH only as compared to the control (distilled water) rats. These markers were significantly increased following co-administration with vitamin C (10 mg/kg & 20mg/kg) respectively. The study also showed that there was no significant difference between the test groups and the control group in hematological parameters.


Conclusion: This finding is indicative of hepatoprotective and antioxidant effect of vitamin C.


Download data is not yet available.

Article Details

How to Cite
B. Maiha, B. ., Bako, A. ., Anafi, S. ., S. Yusuf , M. ., & M. Kabiru, A. . (2020). Amelioration of Isoniazid induced Oxidative Stress and Hematotoxicity by Vitamin C in Wistar Rats . Nigerian Journal of Pharmaceutical and Applied Science Research, 9(4), 23–29. Retrieved from
Author Biographies

Bilkisu B. Maiha

Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria


Sherifat Anafi

Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria

Muhammad S. Yusuf

Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria

Abdulkadir M. Kabiru

Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria


ASHSP, (2006). Isoniazid. The American Society of Health-System Pharmacists. Archived from the original on 20 December 2016. Retrieved 8 December 2016.

Adebayo, A.J., Kehinde, A.J., Adetokunbo, O.A., Olamide, A.E., Oluwatosin, A. (2012) Influence of isoniazid treatment on microsomal lipid peroxidation and antioxidant defense systems of rats. Journal of Drug Metabolism and Toxicology, 3: 120

Aebi, H. (1984). Catalase in Vitro. Methods Enzymology, 105;121-126. (84)05016-3

Ajiboye, J.A., Akintunde, J.K., Oladejo, O., Sand, A., Sabiu, S.A. (2015). Chemoprevention of Silymarin and Vitamin C on Isoniazid-Induced Hepatotoxicity in Experimental Rat Model. Journal of Joxicology, 2(3), 45-50

Atawodi, S. E., Adekunle, O. O. (1989). Antioxidant, organ protective and ameliorative properties of methanol extract anogeissus leiocapus stem bark against carbon tetrachloride-induced liver injury. Intertional Journal of Pharmaceutical Sciences, 2(6), 1443-1448

Bothwell, T.H., Bradlow, B.A and Jacobs, P. (1964) Iron metabolism in scurvy with special to erythropoiesis. British Journal of Haematology. 10: 50-8.

Caro, A.A, Cederbaum, A.I. (2004). Oxidative stress, toxicology, and pharmacology of CYP2E1. Annual Review of Pharmacology and Toxicology, 44: 27–42.

Cheng, J., Krausz, K.W., Li, F., Ma, X., Gonzalez, F.J. (2013). CYP2E1-dependent elevation of serum cholesterol, triglycerides, and hepatic bile acids by isoniazid. Toxicology and Applied Pharmacology; 266: 245–253.

Deng, X., Luyendyk, J.P., Ganey, P.E., Roth, R.A. (2009). Inflammatory stress and idiosyncratic hepatotoxicity: Hints from animal models. Pharmacology Review. 61, 262-282.6.

Elias, A., Oputiri, D., (2013). Hepatoprotective effect of vitamin C. Pharmacology. (4): 84–92.

Ellman, G.L., (1959). Tissue sulphydryl groups. Archive of Biochemistry and Biophysiology. 82: 70?77.

Fridovich, l. (1989). Superoxide dismutase. An adaptation to a paramagnetic gas. Journal of Biochemistry. PMID 2542241. 144 147.

Frieden, TR., Sterling, T.R., Munsiff, S.S., Watt, C.J., Dye, C. (2003). Tuberculosis. MBJ Publishing Group Ltd.

Gini,C.K., Muraleedhara, G.K., (2010). Hepatoprotective effect of Spirulinal on aron paracetamol induced liver damage in rat. Asian Journal of Experimental Biological Science, 1, 6146- 6150.

Hallberg L, Brune M, Rossander L., (1986). Effect of ascorbic acid on iron absorption from different types of meals. Studies with ascorbic acid–rich foods and synthetic ascorbic acid given in different amounts with different meals. Human Nutrition and Applied Nutrition, 40:97-113.

Kedderis, G.L. (1996). Biochemical basis of hepatocellular injury. Journal of Toxicology and Pathology, 24: 77-83.7.

Klaasen C. (2007). Casarett and Doulls toxicity: The basic science of poisons. Mc Graw Hill Professional, New York.

Loulergue, P., Mir, O., Dhote, R., (2007). Pure red bloodcell aplasia and isoniazid use. Emerging Infectious Disease, 13:1427-1428.7.

Means R.T. jr. (2016). Pure Red Cell Aplasia; American Society of hematology. 128(21):2504-2509. doi: 10.1182/blood-2016-05-717140.

Meyer, S.A and Kulkarni, A.P.(2001). Hepatotoxicity. In: Hodgson, E., Smart, R.C. (Eds.). Introduction to biochemical toxicology. 3rd ed. A. New York: John Wiley & Sons, 414-419.

Navarro, V.J., Senior, J.R. (2006). Drug-related hepatotoxicity. New England Journal of Medicine, 354: 731-739.

Ohkawa H. et al. (1979). Assay for lipid peroxides in animal tissues by Thiobarbituric acid reaction. Journal of Analytical Biochemistry. 95(2):351-358. 0.1016/0003-2697(79)90738-3

Osama, A.G., Mahmoud, S.T., Said, A.A., Fatma, A.A., Sanad, Y. (2018). Ameliorative effect of rutin against isoniazid-induced alterations in certain hematological and biochemical parameters of albino rats. International Journal of Pharmacology, 14: 39-51.

Papay, J.I., Clines, D., Rafi, R., Yuen, N., Britt, S.D. (2009). Drug-induced liver injury following positive drug rechallenge. Regulatory Toxicology and Pharmacology. 54: 84-90.4.

Piso, R.J., Kriz K., Desax, M.C. (2011). Severe isoniazid related sideroblastic anemia. Journal Hematology and Repiration. Vol. 3. 10.4081/hr.2011.e2.

Rao, A.V., Balachandran, B. (2002). Role of oxidative stress and antioxidants in neurodegenerative diseases, Nutritional Neuroscience. 5:291-309

Sabiu S., Sunmonu TO., Ajani EO., Ajiboye TO. (2015). Combined administration of silymarin and vitamin C stalls acetaminophen-mediated hepatic oxidative insults in Wistar rats. Brazilian journal of Pharmacology, 25: 29-34.

Sabiu, S., Wudil, A.M., Sunmonu, T.O. (2014). Combined administration of Telfairia occidentalis and Vernonia amygdalina leaf powders ameliorates garlic-induced hepatotoxicity in Wstar rats. Pharmacologia, 5: 191-198

Santhrani, T., Maheswari, E., Saraswathy, G.R., (2012). Amelioration of carbamazepine induced oxidative stress and hematotoxicity by vitamin C. Spatula D2, 173–180.

Santos, L.F., Freitas, R.L., Xavier, S.M., Saldanha, G.B., Freitas, R.M. (2008). Neuroprotective actions of vitamin C related to decreased lipid peroxidation and increased catalase activity in adult rats after pilocarpine- induced seizures. Pharmacology Biochemistry and Behavior, 89:1-5.

Saukkonen, J.J., Cohn, D.L., Jasmer, R.M., Schenker, S., Jereb, J.A. (2006). An Official ATS Statement: Hepatotoxicity of antituberculosis therapy. American Journal of Respiratory and Critical Care Medicine, 174: 935-952.

Sarich, T.C., Adams, S.P., Petricca, G., Wright, J.M. (1999). Inhibition of isoniazid-induced hepatotoxicity in rabbits by pretreatment with an amidase inhibitor. Journal of Pharmacology and Experimental Therapy, 289: 695–702.77.

Shukla, A., Mishra, S., Jain M., Tripathi, A.K. (2014). Pure redcell aplasia: A rare complication of isoniazid therapy. Indian Journal of Hematology and Blood Transfusion, 30: S36- S37.27.

Sminorff, N., Wheeler, G.L. (2000). Ascorbic acid in plants: biosynthesis and function. Critical Review of Biochemical and Molecular Biology, 35: 291- 341.

Stangeland, T., Remberg, S.F., Lye, K.A. (2009). Total antioxidant activity in 35 Ugandan fruits and vegetables. Food Chemistry, 113: 85-91.

Swamy, A.H.M.V., R.V. Kulkarni, A.H.M. Thippeswamy, B.C. Kotiand A. Gore, 2010. Evaluation of hepatoprotective activity of Cissus quadrangularis stem extract against isoniazid-inducedliver damage in rats. Indian Jouranal Pharmacology, 42: 397-400.

World Health Organization. (2008). implementing the WHO Stop TB Strategy: a handbook for national TB control programmes. Geneva: World Health Organization. p. 179.

Willett, K.L., Roth, R.A., Walker, L. (2004). Workshop overview: hepatotoxicity assessment for botanical dietary supplements. Journal of Toxicological Sciences 79: 4-9.

Xavier, S.M., Barbosa, C.O., Barros, D.O., Silva, R.F., Oliveria, A.A. (2007). Vitamin C antioxidant effects in hippocampus of adult Wistar rats after seizures and status epilepticus induced by pilocarpine. Neuroscience Letter, 420: 76-79.

Yue, J., Peng, R.X., Yang, J., Kong, R., Liu, J. (2004). CYP2E1 mediated isoniazid-induced hepatotoxicity in rats. Acta Pharmacologica Sinca a, 25: 699–704.