Antimicrobial evaluation of the methanol crude extract and alkaloid fraction of Persia americana mill seed

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Felix Oghenemaro Enwa
Daniel Johnson Jemikalajah
Micheal Oghenejobo
Iramofu Avwerosuo Akande

Abstract

This study aimed to evaluate the antibacterial property of the methanol crude extract and alkaloid fraction of Persia americana mill seed against Staphylococcus aureus, Streptococcus pyogen, Escherichia coli, Salmonella typhi. The powdered seed of Persia Americana mill was extracted by cold maceration with aqueous methanol (80%v/v). Agar well diffusion method and serial dilution technique were employed in this study. The phytochemical screening conducted on the methanol extract of the plant revealed the presence of flavonoids, saponnins, alkaloids, tannins and carbohyhdrates. The result of the antimicrobial activity of study demonstrated a promising activity against the selected clinical isolates with a more significant zone of inhibition at 500mg/ml. This also revealed that E. coli is resistant to the antibacterial effect of the methanol extract of Persia Americana mill seed at lower concentration (62.5mg/ml and below) and Staph. aureusis more susceptible to the sample at all concentration. Methanol extract of Persia americana mill seed had more antimicrobial activity against Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhi with zone of inhibition of16.5±0.707,18.5±1.121, 11±0.000, 17.5±0.000 respectively compared with alkaloid fraction with zone of inhibition of 13.5±3.536, 14.5±2.120, 0.±0.000, 17.5±2.820. In conclusion, the methanol crude extract and alkaloid fraction showed antimicrobial activity against clinical isolates.

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Enwa , F. O., Jemikalajah , D. J., Oghenejobo , M., & Akande , I. A. (2020). Antimicrobial evaluation of the methanol crude extract and alkaloid fraction of Persia americana mill seed. Nigerian Journal of Pharmaceutical and Applied Science Research, 9(3), 64–70. Retrieved from https://nijophasr.net/index.php/nijophasr/article/view/365
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Author Biographies

Felix Oghenemaro Enwa

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Delta State University, Abraka, Nigeria

Daniel Johnson Jemikalajah

Department of Micrbiology, Faculty of Science, Delta State University, Abraka, Nigeria

Micheal Oghenejobo

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Delta State University, Abraka, Nigeria.

Iramofu Avwerosuo Akande

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Delta State University, Abraka, Nigeria.

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