Antimicrobial Activity of Modified Shrimp Waste-derived Chitosan Films
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Abstract
The addition of different antimicrobial agents to chitosan films has generally enhanced their antimicrobial activity and improved their physical and mechanical properties. The quality of the chitosan films was enhanced by incorporation of red ginger extract into the films. Ginger has the ability to inhibit the growth of pathogenic microorganisms such as bacteria, virus, protozoa and other parasitic organisms. In this work, antibacterial chitosan-starch-glycerol based films incorporated with ginger extract were prepared by thermal gelatinization method. The concentrations of ginger extract in the film forming solutions were varied using different volumes (0.0, 0.5, 1, 1.5 and 2.0 mL) of the extract. FTIR analysis was carried out in order to assess the functional group interactions between the matrix and the added agents. The antimicrobial activities of the modified chitosan films, carried out using Disc Diffusion method, showed that films incorporated with 2 mL of ginger extract had the largest zones of inhibition against Staphylococcus aureus and Pseudomonas aeruginosa compared to the other films. Modified chitosan films without ginger extract showed minimal antimicrobial activities, while films with neither chitosan nor ginger extract showed no antimicrobial activities.
Keywords: Chitosan, edible film, ginger extract, antimicrobial
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