Effect of Disulphide Derivative of Dihydroartemisinin on the Haematological Indices of N-Methyl-N-Nitrosourea Injected Rats.
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Abstract
Dihydroartemisinin (DHA) possesses anticancer activity but limited by its short half-life. Recently, a novel cancer chemotherapeutic agent, a disulphide derivative of DHA (sDHA) has been synthesized with the aim of extending its half-life. N-methyl-N-nitrosourea (MNU) has been largely used to induce cancer in the study of a variety of novel cancer chemotherapeutic agents. MNU induced carcinogenesis causes alterations in haematological parameters. This study evaluates the effect of sDHA on the haematological indices of MNU injected rats. MNU (50 mg/kg) was administered intravenously as single dose to different animal groups (n=10), followed ten days later by oral treatment with sDHA (37.42, 74.83 and 112.25 mg/kg), Tween 80 (0.3 ml) and distilled water (0.3 ml) once daily for 28 days. Animals were observed for 52 days after drug treatments after which the animals were sacrificed and blood collected for assay of haematological parameters [Hb, RBC, PCV, MCH, MCHC, PLT, WBC and WBC differentials (eosinophils, neutrophils, lymphocytes, monocytes and basophils)]. MNU significantly (p<0.0001) increased serum levels of WBC and differentials and decreased the levels of all red blood indices studied. Oral treatment with sDHA resulted in significant (p<0.001) dose-dependent reductions in the serum levels of MNU-induced elevations of WBC and differentials and increased MNU-induced reduction of PCV and PLT levels in a dose-dependent manner, and that of RBC, Hb, MCH and MCHC at the highest dose administered (112.25mg/kg). The study shows that sDHA has the potential to ameliorate the MNU-induced alterations in haematological indices in rats.
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